摘要
目的观察燃煤型氟中毒大鼠脑组织中斯钙素蛋白（STC）1及钙离子的变化，探讨STCl在燃煤型氟中毒脑损伤机制中的作用。方法24只雄性SD大鼠按体质量随机分为4组：对照组、低、中、高氟组；对照组采用常规大鼠饲料（含氟1．3mg／kg），低、中、高氟组分别饲以含氟为20．0、40．0、60．0mg／kg的饲料，所有大鼠均饮用蒸馏水，自由饮食、饮水；180d后，采用免疫组化和RT—PCR方法，检测大鼠脑组织中STCl蛋白及基因的表达，并检测脑组织内钙离子含量。结果与对照组[（24．70±3．53）％]相比，低、中、高氟组大鼠STCl细胞阳性率[（48．10±2．11）％、（54．90±1．73）％、（79．30±3．71）％]均显著增高（P〈0．05），其中高氟组明显高于低、中氟组（P均〈0．05）。低、中、高组大鼠脑组织中STCl的mRNA表达（0．58±0．09、0．85±0．17、1．75±0．04）均高于对照组（0．37±0．12，P〈0．05），高氟组STClmRNA表达高于低、中氟组（P均〈0．05）。低、中、高氟组大鼠脑皮质钙离子浓度[（138．62±4．19）、（167．43±6．57）、（189．45±3．72）nmol／L]显著高于对照组[（101．47±9．46）nmol／L，P均〈0．05]，其中高氟组高于低、中氟组（尸均〈0．05），中氟组高于低氟组（P〈0．05）。结论STCl可能依靠调节钙离子平衡的功能参与了燃煤型氟中毒脑损伤机制。
Objective To observe the change of stanniocalcin 1 （STC1） and calcium content in brain of coal-burning-borne fluorosis rats, and to explore the role of STC1 in brain injury of coal-burning-borne fluorosis. Methods Twenty four male SD rats were randomly divided into control, low, medium, and high fluoride groups according to body mass. Control group was fed conventional rat chow（fluorinated 1.3 mg/kg）, and low, medium and high fluoride groups fed with fluorinated feed（20.0, 40.0, 60.0 mg/kg）. All rats were given distilled water and feed ad libitum. One hundred and eighty days after modeling, STC1 protein and gene expression in the brain tissue of rats were detected using immunohistochemistry and RT-PCR and calcium content of brain tissue was detected. Results The cell positive rates of STCI in low, medium, high fluoride groups [ （48.10 ± 2.11 ）%, （54.90 ± 1.73）%, （79.30± 3.71）%] were significantly higher than that of the control group[（24.70 ± 3.53）%, all P 〈 0.05], the cell positive rate of high fluoride group was significantly higher than that of the low and medium fluoride groups （all P 〈 0.05）. The STC1 mRNA expression of tow, medium and high fluoride groups （0.58 ± 0.09, 0.85 ± 0.17, 1.75 ± 0.04） were significantly higher than that in the control group（0.37 ± 0.12, all P 〈 0.05）, the STC1 mRNA expressions of high fluoride group was significantly higher than that of the low and medium fluoride groups （all P 〈 0.05 ）. The brain cortex calcium ion concentrations of low, medium and high fluoridegroups[（138.62± 4.19）, （167.43 ± 6.57）, （189.45 ± 3.72）nmol/L] were significantly higher than that in the control group [ （101.47 ± 9.46）nmol/L, all P 〈 0.05 ], the brain cortex calcium ion concentrations of high fluoride group was significantly higher than that of the low and medium fluoride groups（all P 〈 0.05）, and the medium fluoride groups was higher than the low groups （P 〈 0.05 ）. Conehmion STC1 may be involved in brain damage of coal-burning-borne fluorosis rats through regulating calcium balance.
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2013年第2期129-132,共4页
Chinese Journal of Endemiology
基金
国家自然科学基金青年项目（11102235）
天津市自然科学基金重点项目（12JCZDJC24100）
武警医学院科研基金面上项目（WYM201118）
武警医学院科研基金博士启动金项目（WYB201110）
关键词
氟中毒
牙
脑损伤
斯钙素蛋白1
钙
Fluorosis, dental
Brain injuries
Stanniocalcin 1
Calcium
