摘要
目的探讨铝对氟中毒小鼠斑状脱毛的保护作用，氟对小鼠皮肤的损伤及铝对氟毒性的拮抗作用。方法60只雄性C57／BL小鼠按体质量随机分为对照组、氟组（氟离子100mg／L）、铝组（铝离子270mg／L）和氟＋铝组（氟离子100mg／L＋铝离子270mg／L），每组15只，分别在饲养1、3个月时处死取骨组织。离子选择电极法检测骨氟含量；电感耦合等离子体发射光谱法测定骨铝水平；观察小鼠氟斑牙和脱毛发生情况。结果饲养1个月时，各组小鼠均无氟斑牙和脱毛现象发生；氟组、氟＋铝组、对照组、铝组骨氟依次降低，分别为（2401．6494±86．835）、（1210．3324±19．531）、（538．0014±33．337）、（427．0064±11．878）mg／kg，任意两组组间比较，差异均有统计学意义（P均〈0．05）。饲养3个月时，氟组10只小鼠均发生氟斑牙和斑状脱毛，脱毛区域位于颈部和背部，其他体表部位未见脱毛现象，而对照组、铝组和氟＋铝组小鼠均无脱毛现象，并且对照组和铝组无氟斑牙发生，氟＋铝组仅有2例轻度氟斑牙发生；氟组、氟＋铝组、对照组、铝组骨氟从高到低依次为（4098．6454±58．842）、（1888．1654±12．187）、（876．2584±14．462）、（662．385±8．966）mg／kg，任意两组组间比较，差异均有统计学意义（P均〈0．05）。结论氟中毒小鼠有脱毛现象发生，小鼠脱毛与氟暴露程度密切相关；铝通过减少氟在体内的蓄积而预防染氟小鼠脱毛的发生。
Objective To investigate the effect of aluminum on hair loss induced by fluoride in fluorosis mice. Methods Sixty male C57BL mice were divided into four groups according to body mass： control group, fluoride （F） group （F- 100 mg/L）, aluminum（A1） group（A13＋ 270 mg/L） and F ＋ A1 group（F- 100 mg/L ＋ A13＋ 270 mg/L）. Mice were killed 1 month and 3 months after the experiment, respectively. Bone F content was detected by ion-selective electrode method. The level of bone A1 was measured through inductively coupled plasma emission spectrum. Dental fluorosis and hair loss of mice were evaluated by visual method. Results One month after the experiment, no dental fluorosis and hair loss was found in all four groups. The content of bone F was the highest in F group[ （2401.649 ± 86.835）mg/kg], and the lowest in A1 group [ （427.006 ± 11.878）mg/kg]. The levels of bone F in F ＋ A1 group and control group were （1210.332± 19.531）mg/kg and （538.001 ± 33.337）mg/kg, respeetively. The difference was statistically significant between any two groups （all P 〈 0.05 ）. Three month after the experiment, all mice of F treatment group had dental fluorosis and hair loss（10/10）. Alopeeia areas were found in the neck and back regions only. There was no hair loss in control group, A1 group and F ＋ A1 group. No dental fluorosis was found in both eontrol and A1 groups. Only 2 mice were found with dental fluorosis in F ＋ A1 group. The levels of bone F in F group, F ＋ A1 group, control group and A1 group were （4098.645 ± 58.842）, （1888.165 ± 12.187 ）, （876.258 ± 14.462） and （662.385± 8.966） mg/kg, respectively. The differerice was statistically significant between any two groups （all P 〈 0.05）. Conclusions The hair loss is found in fluorosis mice. Hair lossof mice is closely associated with the level of F exposure. A1 can prevent the occurrence of hair loss induced by F in mice through reducing the accumulation of F.
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2013年第2期136-139,共4页
Chinese Journal of Endemiology
基金
国家自然科学基金（81072252）
关键词
氟中毒
牙
铝
毛发
Fluorosis, dental
Aluminum
Hair
