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甘蔗无根试管苗移栽生根过程中内源激素和POD活性变化的研究 被引量:4

The endogenous hormone and POD activity changes in ex vitro rooting of sugarcane microshoots in transplanting period
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摘要 以新台糖22号(ROC22)甘蔗无根试管苗为材料,将其移栽至苗圃中,对其生根过程中根发生情况以及内源激素吲哚乙酸(IAA)、二氢玉米素(zR)和脱落酸(ABA)含量以及过氧化物酶(POD)活性进行研究。结果表明:根突破表皮的时间为4~6d.6d时80%幼苗长出可见根。内源激素IAA、ABA、ZR的含量在根原基诱导阶段逐步上升(0~2d);在根原基形成阶段逐渐下降,并处于较低水平(2~4d);在根突破表皮前后又逐渐升高(4~8d)。POD活性在根原基诱导阶段逐渐下降:根原基形成阶段波动很大,出现一个最低点和一个最高点;根突破表皮前活性降低,根生长阶段处于稳定水平。 Microshoots of sugarcane cultivar ROC22 were used to study root occurrence and changes in the contents of IAA, ZR and ABA and the activities of POD in the course of root development from root primordial induction to root elongation after those mieroshoots without root system have been transplanted in the green house. The results showed that the root germination occurred at 4-6 d, and 80% of plantlets were observed visible roots at 6 d. The contents of IAA, ZR and ABA increased at root primordia-induetion(0-2 d), decreased and kept at low level at root primordial formation (2-4 d) and increased before and after root germination (4-8 d). The activity of POD decreased at root primordial induction, fluctuated from minimum point to maximum point at root primordial formation, decreased before root germination and kept at low and stable level during root elongation.
出处 《广东农业科学》 CAS CSCD 北大核心 2013年第3期15-17,31,共4页 Guangdong Agricultural Sciences
基金 广西科学研究与技术开发项目(桂科合140011-3) 中央级公益性科研院所基本科研业务费项目(G2009013) 农业部广西甘蔗生物技术与遗传改良重点实验室科研专项(12-071-30) 广西重点实验室建设项目(12-A-04-01) 广西农业科学院基本科研业务费专项(桂农科2012YM11)
关键词 甘蔗 无根试管苗 内源激素 过氧化物酶活性 sugarcane microshoots endogenous hormone POD activity
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