摘要
采用PMSF、PCMB、NBS、TNBS、SUAN、DTT、IAc、BrAc等化学修饰剂在一定的条件下选择性修饰罗非鱼ALP,研究罗非鱼ALP分子中氨基酸侧链基团与酶活性中心的关系。结果表明:PMSF、NBS等两种化学修饰剂能显著抑制酶的活力,而PCMB、TNBS、SUAN、DTT、IAc、BrAc等对酶的活力影响不大。说明对丝氨酸残基、色氨酸残基的化学修饰后对酶的活力影响很大,而酶分子中的其他功能基团不在酶的活性中心,修饰后对酶的活力影响不大。
The alkaline phosphatase from Oreochromis niloticus （Tilapia） were selectively modified by chemical reagents such as PMSF, PCMB, NBS, TNBS, SUAN, DTT, IAc and BrAc respectively. It could be found that the enzyme activity was significantly decreased after modification of PMSF, NBS, but oppositely the PCMB, TNBS, SUAN, DTT, IAc, BrAe didn＇t obviously effect the alkaline phosphatase enzyme activity. Studied of the chemical modification demonstrated that the Tryptophan residues and Serine residues were essential functional groups inside of the alkaline phosphatase active site, the same time suggested that the others residues were not located in the enzyme active site, didn＇t directly effect the enzyme activity.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第3期82-84,共3页
Guangdong Agricultural Sciences
基金
贵州省黔西南州科技局资助项目(2011-9)
关键词
罗非鱼
碱性磷酸酶
化学修饰
Oreochromis niloticus
alkaline phosphatase
chemical modification
