摘要
了解和掌握2009～2011年湖南省甲型H1N1流感流行动态和变化规律,掌握甲型H1N1流感流行株基因特性及耐药性情况。收集哨点医院采集的流感样病例咽拭子标本,通过荧光PCR法或病毒分离法对流感病毒进行检测,选取部分阳性毒株进行基因序列测定,序列使用MEGA 5.05软件完成进化分析。2009年第20周至2011年52周,共检测标本17 773份,检出流感阳性标本3 831份,检测阳性率为21.6%,其中甲型H1N1流感阳性标本1794份,占流感阳性的比例为46.8%。甲型H1N1流感共有2个流行高峰,分别出现在2009年第41～53周和2011年第1～12周。测序的23株毒株HA基因亲缘关系较近,病毒在基因进化树中基本上按照时间顺序分布。全基因组序列分析显示7株毒株的所有8个基因片段均与疫苗株同源,并未发现基因重配。23株毒株的HA氨基酸位点相对于疫苗株高度相似(同源性为98.2%～100%),但均有P83S、S203T和I321V的突变。在A/Hunan/YQ30/2009毒株中发现了可能导致病毒毒力增强的222位点突变,突变为D222E。所有检出毒株均未发现对奥斯他韦耐药性的突变。2009～2011年湖南省甲型H1N1流感流行呈双峰分布,未发现病毒基因发生大规模变异,临床上使用奥斯他韦仍然是有效的。
To understand and master the dynamic variation of the pandemm influenza A （H1N1） 2009 in Hunan province from 2009 to 2011, and to know the genetic characteristics and drug resistance of the pandemic （HIN1） 2009 viruses. Throat swab specimens of influenza-like illness patients were collected from sentinel hospitals and tested for influenza by fluorescent PCR or virus isolation methods. Partial iso-lates were selected for sequencing. The sequences were used for phylogenetic analysis by MEGA 5.05 soft-ware. From the 20th week of 2009 to the 52nd week of 2011, 17 773 specimens were tested. 3 831 specimens were influenza-positive with a positive rate of 21.6%, of which 1 794 were positive specimens of pandemic （HIN1） 2009, accounting for 46.8% of the influenza-positives. There were 2 epidemic peaks of pandemic （HIN1） 2009, which were in the 41st-53rd week of 2009 and the 1st-12nd week of 2011, respec- tively. The HA genes of 23 strains that were selected for sequencing had close relationship; the distribution of strains in the phylogenetic tree was basically in chronological order. The complete genome sequence analysis showed that all of 8 gene segments of 7 strains were homologous to the vaccine strain, and there was no gene reassortment. The HA amino acid sites of the 23 strains were highly similar to the vaccine strain （98. 2%-100.0% in homology）, but all 23 strains had P83S, S203T and I321V mutations. The 222 site mutation that may lead to enhanced virulence was found in the A/Hunan/YQ30/2009 strain. The mutation was D222E. There was no oseltamivir resistance mutation found in all strains. The pandemic （H1N1） 2009 in Hunan province from 2009 to 2011 had a bimodal distribution. There was no large-scale variation of virus genes. The clinical use of oseltamivir was still effective.
出处
《病毒学报》
CAS
CSCD
北大核心
2013年第2期148-153,共6页
Chinese Journal of Virology
基金
国家"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项课题(2008ZX10004-013)
湖南省科技厅项目(2010ZK3034)
湖南省卫生厅重点项目(A2007008)
关键词
甲型H1N1流感
监测
基因特性
Pandemic （H1N1） 2009
Surveillance
Genetic characteristics
