摘要
了解内蒙古乌兰察布市集宁地区鸡新城疫的流行概况,对该地区疑似新城疫病毒(NDV)感染的病料进行分离鉴定。通过接种10日龄SPF鸡胚,结果分离到2株病毒,经血凝试验及新城疫和禽流感阳性血清的血凝抑制试验,证明2个分离株均为新城疫病毒,分别命名为JN1、JN2,并对分离的2个毒株进行MDT、ICPI和IVPI毒力测定,结果表明,分离株的MDT分别为40.5h和43.5h,ICPI分别为1.86和1.85,IVPI分别为2.38和2.69。可以判定JN1和JN2病毒株为新城疫病毒强毒株。
In order to study the prevalence in Jining Wulanchabu of Inner Mongolia Newcastle disease, the suspected chicken samples were collected from the area. Newcastle disease virus （NDV） was isolated, through the passage of the 10-day-old SPF chicken embryo inoculation. The two isolates were obtained,and the hemagglutination and Newcastle disease virus and Avian influenza virus-positive serum hemagglutination inhibition tests determined the two isolates are Newcastle disease virus, named for JN1 JN2. The MDT, ICPI and IVPI virulence of two isolates were MDT 40.5 h and 43.5 h, 1.86 and 1.85,2.38 and 2.69, respectively. JN1 and JN2 two isolates are virulent NDV strains.
出处
《动物医学进展》
CSCD
北大核心
2013年第4期129-132,共4页
Progress In Veterinary Medicine
关键词
新城疫病毒
分离鉴定
生物学特性
Newcastle disease virus
isolation and identification
biological characteristic
