摘要
目的 :研究长期运动训练对老年小鼠骨骼肌线粒体复合物I和复合物Ⅳ活性的影响 ,并探讨其机制。方法 :以C57BL/6J雄性小鼠跑转笼为运动方式 ,通过分光光度法和极谱氧电极法测定线粒体复合物I和复合物Ⅳ的活性。结果 :随着小鼠年龄的增长 ,骨骼肌线粒体复合物I(NADH脱氢酶)活性显著下降 ,复合物IV(细胞色素氧化酶 )活性无明显变化。经过8个月运动训练的小鼠骨骼肌线粒体复合物I及复合物IV活性明显升高 ,显著高于同龄对照鼠甚至高于5月龄鼠。结论 :运动训练在一定程度上致骨骼肌线粒体功能产生适应性变化。
Objective:To observe the effects of long term physical activity (running wheel) on the age-related changes of complex I and IV activities of mice skeletal muscle mitochondria. Methods:The activity level of complex I and complex Ⅳof 10 elderly training mice,10 elder non-training mice and 10 young mice was obtained by spectrophotography and polarography.Result: The complex I activity decreased with age, and complex IV activity was not influenced. After 8 months physical training, the activities of complex I and IV increased.Conclusion:The adaptive changes of mitochondria were brought by long term physical activity which might delay the age-related decline of mitochondrial function.
出处
《现代康复》
CSCD
2000年第4期568-569,共2页
Modern Rehabilitation
关键词
运动训练
骨骼肌线粒体
复合物Ⅰ
复合物Ⅳ
physical activity
skeletal muscle mitochondria
complex I
complex IV
