摘要
目的通过检测牛乳β乳球蛋白免疫反应性,分析其空间结构的变化,以探讨过敏原免疫反应性下降的可能机制。方法分别取牛乳β乳球蛋白置于空气净化器(接受1、12、24h的作用,实验组)、与实验组相同温度和湿度的环境(12、24h后取样,对照组)、4℃冰箱(正常组)中。用ELISA法检测牛乳β乳球蛋白免疫反应性,采用圆二色谱仪和荧光光谱仪检测空气净化器处理前后的牛乳β乳球蛋白,分析牛乳β乳球蛋白空间结构的变化。结果实验组(作用24h)的光密度值接近0.88,相对于正常组的光密度值1.27和对照组的光密度值1.25,降低了约30%。牛乳β-乳球蛋白在空气净化器作用前后,正常组和对照组的圆二色谱光谱曲线没有发生明显的变化,而实验组处理12h和处理24h负峰值与正常组相比有明显的升高,并且呈现一定的递增趋势。实验组在280nm和295nm处的荧光强度明显强于正常组和对照组,且随着处理时间的增加强度越强。结论经过空气净化器处理之后的牛乳β乳球蛋白免疫反应性有所降低,圆二色谱光谱实验和荧光光谱实验结果表明其空间结构发生了变化,空间结构变化可能是使其免疫反应性改变的原因。
Objective Through detecting the immunogenicity of β-lactoglobulin and analyzing the space structure,the mechanism that immunogenicity of allergens decreases is discussed in thispaper. Methods Experimental group （EP）.β-lactoglobulin was treated by air cleaner for 1 h, 12 h,and 24 h. Control group （CP）：β-lactoglobulin was placed in the same temperature and hu-midity with the experimental group. Normal group （NP） ：β-1actoglobulin was placed at 4 ℃. The immunogenicity of β-1actoglobulin was detected by ELISA. β-1actoglobulin was detected beforeand after treated by air cleaner,and the space structure was analyzed. Results The OD value of experimental group after treated for 24 h was nearly 0. 88 , reducing about 30 % compared with the1.27 of NP and 1.25 of CP. Before and after treated by air cleaner,the curves of β-lactoglobulin unchanged in NP and CP. However,negative peak of EP （12 h and 24 h） obviously increased andpresented an increasing trend compared with the NP. The fluorescence intensity of EP was clearly stronger than NP and CP at 280 nm and 295 nm,and increased as the treating time increased. Conclusion After treated by air cleaner, the immunogenicity of β-lactoglobulin decreased, and through analysis of circular dichroism and fluorescence spectroscopy,the space structure of β-lactoglobulin varied. Change of space structure maybe the reason leading to the changes of immunogenicity.
出处
《南昌大学学报（医学版）》
CAS
2013年第1期1-4,12,共5页
Journal of Nanchang University：Medical Sciences
基金
国家863计划(2006AA02A231)
国家自然科学基金(81071388
30760082)
深圳市重点实验室组建项目(SW201110010)
深圳大学基础研究科研项目
