摘要
目的探讨绿茶提取物(EGCG)对大鼠糖尿病肾病模型氧化应激、肾损害、肾功能以及纤维化相关因素的治疗作用。方法取雄性、8～10周龄、体质量210～230g的Wistar大鼠60只,高糖高脂饲料喂养4周。将60只大鼠按随机数字表法分为正常对照组(A组,n=16)、糖尿病模型组(n=44)。糖尿病模型组一次性腹腔内注射链脲佐菌素(STZ)30mg·kg-1,48h后测血糖,将39只存活、成模的大鼠按随机数字表法分为糖尿病肾病模型组(B组,n=20)和糖尿病肾病模型治疗组(C组,n=19)。A、B、C组大鼠进行高蛋白饲料喂养,C组给予EGCG1g·d-1。在第12周进行血糖、糖化血红蛋白(HbA1c)、血肌酐(SCr)、尿素氮(BUN)、24h尿蛋白定量、尿β2-微球蛋白(β2-MG)测定,ELISA法测定血清TGF-β1,荧光分光光度法测定血清AGEs水平,肾脏病理检查、免疫组织化学检测肾组织TGF-β1、MCP-1、RAGE的表达。结果 3组大鼠血糖、HbA1c、SCr、BUN、24h尿蛋白定量、尿β2-MG、血清TGF-β1、血清AGEs水平差异均有统计学意义(均P<0.05),且C组均明显低于B组(P<0.05);C组肾脏病理损害明显轻于B组,肾组织中TGF-β1、MCP-1、RAGE的表达也明显少于B组。结论 EGCG能减轻大鼠糖尿病肾病模型肾损害、下调肾脏纤维化相关因素的表达,其作用机制可能与拮抗氧化应激有关。
Objective To investigate the effect of （-）-epigallocatechin 3-O-gallate（EGCG） on oxidative stress,renal damage, renal function and fibrosis-related factors in rats with diabetic he-phropathy. Methods Sixty male Wistar rats （8-10 weeks old and 210-230 g） fed a high-fat diet for 4 weeks were randomly divided into two groups.normal control group （group A,n= 16） anddiabetic model group （n = 44）. Rats in diabetic model group were intraperitoneally injected with streptozocin （30 mg·kg^-1）. Blood glucose levels were determined after 48 hours. Thirty-ninesurvival rats with diabetic nephropathy were randomly divided into diabetic nephropathy group （group B,n=20） and EGCG treatment group （group C,n= 19）. All rats were fed a high-proteindiet. Rats in group C were additionally given EGCG （1 g·d^-1）. Levels of fasting plasma glucose, glycosylated hemoglobin （HbAlc）, serum creatinine （SCr）, blood urea nitrogen （BUN）, 24-hoururinary protein excretion and urinaryβ2-MG were measured at the 12th weeks. The serum AGEs and TGF-β1 were determined by fluorospectrophotometry and ELISA, respectively. The renalpathological changes were examined and the expression of TGF-β1, MCP-1 and RAGE in nephridi-al tissue was detected by immunohistochemical staining. Results There were significantly differences among group A, group B and group C in levels of fasting plasma glucose, HbAlc, SCr,BUN, 24-hour urinary protein excretion, urinaryβ2-MG, AGEs and TGF-β1 （P〈0.05）, and EGCG treatment obviously improved these parameters in rats with diabetic nephropathy （P 〈0. 05）.Compared with group B,renal damage and expression of TGF-β1 ,MCP-1 and RAGE significantly decreased in group C. Conclusion The treatment with EGCG can reduce renal damage and decrease the expression of fibrosis-related factors in rats with diabetic nephropathy through inhibiting oxidative stress.
出处
《南昌大学学报（医学版）》
CAS
2013年第1期21-25,共5页
Journal of Nanchang University：Medical Sciences
基金
广东省中医药局计划项目(20110230)
深圳市科技局计划项目(201103238)
关键词
糖尿病肾病
绿茶提取物
氧化应激
动物
实验
大鼠
diabetic nephropathy
（-）-epigallocatechin 3-O-gallate
oxidative stress
animals, laboratory
rats
