摘要
利用电子克隆技术从木薯基因组DNA中获得1个MADS-box基因,对其理化性质进行分析,并对该蛋白响应激素信号进行研究。结果表明:该基因含有全长为687 bp的ORF,编码229个氨基酸。通过对该蛋白进行ProtScale程序预测表明,该蛋白可能为亲水性蛋白;理化性质分析表明,该蛋白相对分子量为26.103 7 ku,等电点为6.87;跨膜结构域预测表明,该蛋白为非分泌型蛋白;亚细胞定位分析该蛋白可能定位于细胞核;磷酸化位点分析表明该蛋白能被丝氨酸激酶所磷酸化。运用定量PCR对该基因响应乙烯信号的表达模式进行分析,结果表明,该基因能够响应乙烯信号,并在外施乙烯12 h时具有最高的表达量。本研究为进一步研究该基因响应乙烯信号影响木薯叶片的生长发育提供研究基础。
A Cassava MADS-box nucleotide sequence was acquired by in silico cloning method. And the gene was cloned from cassava genomic DNA with PCR. The results of bioinformatics analysis indicted that the cassava MADS-box gene has a complete ORF with a long of 687 bp, and encoded a protein with 229 amino acids, ProtScale predict indicted that this protein was a hydrophilic protein, physicochemical property analysis suggested that this protein was 26.103 7 ku and had a PI of 6.87, signal peptide predication showed that this protein was a non-secretary protein, sub-cellular localization analysis showed that this protein maybe locate in nucleus, phosphorylation site analysis indicted that this protein could be catalyzed by PKC, CKI, PKG Kinesis, and the expression patterns analysis response to ethylene treatment indicted that this gene maybe response to ethylene. This research provides a research foundation for further studying the response to ethylene signaling and its function on affecting the growth and development of cassava.
出处
《热带农业科学》
2013年第2期30-35,共6页
Chinese Journal of Tropical Agriculture
基金
国家国际科技合作专项项目"木薯离区叶片脱落机制和高产抗旱新品系合作开发"(No.2013DFA32020)
国家863项目"重要热带作物木薯定向分子设计育种与种质创新"(No.2012AA101204-2)
