摘要
原子力显微镜(AFM)是生物显微技术的一个重要组成部分,可实现液体环境下活细胞高分辨率的成像与操作,为细胞生物学研究提供了新的方法。近年来,AFM已经逐渐发展成为集样品成像、力测量及操作等功能于一体的多功能生物细胞研究平台,在细胞研究中得到了广泛的应用。在简要介绍AFM组成与工作原理的基础上,详细阐述了近年来基于AFM成像与力谱技术的细胞研究的发展状况。针对AFM本身所存在的不足,介绍了AFM与其他技术相结合的研究成果,并对AFM在生物细胞研究中未来的发展方向进行了展望。
Atomic force microscopy （AFM） is an important part of the biological microscope tech- nology, which can realize high resolution imaging and manipulating of living cells in liquid envi ronment, and provide a new method for the cell biology research. In recent years, AFM has gra- dually developed into a multi-function biological cell research platform with the functions of the sample imaging, force measurement and operation, which has been widely used in the cell re- search. On the basis of the brief introduction of AFM configuration and working principle, the cell research developments based on the AFM imaging and force spectrum technology are dis- cussed in detail. For covering the shortages of the AFM, the research results of AFM combined with other technologies are introduced, and the development trends of the AFM in the cell biolo- gy research are prospected.
出处
《微纳电子技术》
CAS
北大核心
2013年第4期248-254,共7页
Micronanoelectronic Technology
基金
国家自然科学基金资助项目(50905105)
机器人技术与系统国家重点实验室开放课题资助项目(SKLRS-2010-MS-02)
