摘要
目的:研究更年轻片对去势更年期模型大鼠促卵泡激素(FSH)、黄体生成素(LH)、泌乳素(PRL)、血清雌二醇(E2)的影响及对小鼠的镇静作用。方法:通过手术去除SD雌性大鼠双侧卵巢以复制大鼠去势更年期模型。实验分为正常(等容生理盐水)、模型(等容生理盐水)、倍美力结合雌激素(104.2mg/kg)与更年轻高、中、低剂量(1040、520、260mg/kg)组,灌胃给药,每天1次,连续30d。观察大鼠给药前后体质量变化,测定血清中FSH、LH、PRL、E2水平,计算子宫系数和阴道上皮细胞角化率。50只雌性小鼠随机均分为空白(等容生理盐水)、安定片(5mg/kg)与更年轻高、中、低剂量(2080、1040、520mg/kg)组,灌胃给药,每天1次,连续5d。于末次给药后1h测定5min内小鼠的活动次数和站立次数。50只雌性小鼠随机均分为空白(等容生理盐水)、安定片(5mg/kg)与更年轻高、中、低剂量(2080、1040、520mg/kg)组,灌胃给药,每天1次,连续6d。于末次给药后1h腹腔注射戊巴比妥钠(40mg/kg),记录各组小鼠的睡眠潜伏期和睡眠持续时间。结果:与模型组比较,更年轻高、中、低剂量组FSH、LH和PRL水平明显降低,E2水平明显升高;阴道上皮细胞角化率明显提高;子宫系数明显增加。与空白组比较,更年轻高、中、低剂量组小鼠的活动次数和站立次数显著减少(P<0.05);睡眠时间明显延长。结论:更年轻片对去势更年期模型大鼠有一定的疗效,对小鼠有一定的镇静催眠作用。
OBJECTIVE： To observe the effect of Gengnianqing tablets on FSH, LH, PRL and E2 in ovariectomized menopausal model rats and the sedation of mice. METHODS： Ovariectomized menopausal model of SD female rats were induced by removal of bilateral ovaries. SD rats were randomized into 6 groups, i.e. normal group （constant volume of normal saline）, model group （constant volume of normal saline） , premarin combined with female hormone group （104.2 mg/kg） and Gengnianqing tablets high-dose, medium-dose and low-dose group （ 1 040, 520, 260 mg/kg） groups. They were given relevant medicine intragastrically once a day for consecutive 30 days.The changes of rats＇ body weight were observed before and after administration, and serum levels of FSH, LH, PRL and Ez were determined. The uterine coefficient and the rate of vaginal epithelial cellular keratinization both were calculated. 50 female mice were randomized into blank group （constant volume of normal saline） , Diazepam tablet group （5 mg/kg） and Gengnianqing tablets high-dose, medium-dose and low-dose groups （2 080, 1 040, 520 mg/kg）. They were given relevant medicine intragastrically once a day for consecutive 5 days.The number of stand-up and activities in mice within 5 rain were determined 1 h after the last administration. 50 female mice were randomized into blank group （constant volume of normal saline）, Diazepam tablet group （5 mg/kg） and Gengnianqing tablets high-dose, medium-dose and low-dose groups （2 080, 1 040, 520 mg/kg）. They were given relevant medicine intragastrically once a day for consecutive 6 days. 1 h after last administration, they were given pentobarbital sodium （40 mg/kg） intraperitoneally, the mice ＇sleep latency and sleep duration were recorded in each group. RESULTS： Compared with model group, the levels of FSH, LH and PRL were decreased significantly in Gengnianqing tablets high-dose, medium-dose and low-dose groups, but the level of E2 was increased. The rate of vaginal epithelial cellular keratinization was increased and the uterine factor was increased too. Compared with blank group, the number of stand-up and activities in mice were decreased significantly, and sleep duration extended significantly. CONCLUSION： Gengnianqing tablets have a certain effect on ovariectomized menopausal model rats, and have sedative and hypnotic effects on mice.
出处
《中国药房》
CAS
CSCD
2013年第15期1365-1368,共4页
China Pharmacy
关键词
更年轻片
更年期障碍
子宫系数
镇静催眠
Gengnianqing tablets
Menopausal disorder
Uterine factor
Sedative-hypnotic
