摘要
目的制备甘草次酸固体脂质纳米凝胶并考察其体外透皮效应。方法采用微乳液法制备甘草次酸固体脂质纳米粒并考察其包封率、粒径与表面电位,以研和法制备固体脂质纳米粒凝胶;采用改良Franz立式扩散池法进行体外透皮实验,HPLC法测定甘草次酸含量,评价甘草次酸固体脂质纳米粒凝胶的经皮渗透结果。结果甘草次酸固体脂质纳米粒外观为圆球形或椭球形;甘草次酸固体脂质纳米粒的包封率为64.75%±1.36%,粒径范围(46.13±20.10)nm,电位分布范围为(-53.4±7.11)mV。24h甘草次酸固体脂质纳米粒凝胶较甘草次酸固体脂质纳米粒的累积透过量提高66%。结论甘草次酸固体脂质纳米粒凝胶能提高甘草次酸的透皮速率,有望成为甘草次酸透皮给药的新型制剂。
Objective To prepare glycyrrhetinic acid solid lipid nanoparticle gels（GA-SLNsG）,and to investigate its transdermal delivery in vitro.Methods GA-SLNsG was prepared based on the microemulsion technique,and its entrapment efficiency,size and Zeta potential were determined;the glycyrrhetinic acid solid lipid nanoparticle gels was prepared by mixing method.The transdermal delivery in modified Franz diffusion cells for isplated rat shin of GA-SLNs,GA gel and GA-SLNsG were studied respectively.The amount of penetrated glycyrrhetinic acid was determined by HPLC.Results GA-SLNs were spherical or ellipsoidal,and its encapsulation efficiency was 64.75%±1.36%,particle size was（46.13±20.10） nm,and Zeta potential was（-53.4±7.11） mV.The accumulative penetrated amount of glycyrrhetinic acid of GA-SLNsG was 66% higher than that of GA-SLNs within 24 hours in vitro.Conclusions Glycyrrhetinic acid solid lipid nanoparticle gels improved the glycyrrhetinic acid transdermal delivery rate and it has promising development value as an effective transdermal delivery formulation for glycyrrhetinic acid.
出处
《西北药学杂志》
CAS
2013年第2期197-200,共4页
Northwest Pharmaceutical Journal
关键词
甘草次酸
固体脂质纳米粒
凝胶
体外透皮
glycyrrhetinic acid
solid lipid nanoparticles
gels
transdermal delivery
