摘要
目的 探讨测定葛根芩连袋泡剂质量标准的方法 方法 本实验采用薄层层析法对葛根芩连袋泡剂的主要中药葛根、黄芩及黄连进行了定性分析 ,并通过荧光淬灭薄层扫描法测定了君药———葛根中葛根素的含量 .结果 袋泡剂中葛根素平均含量为 0 .0 776 % (g/ 10 0g) ;加样提取平均回收率为 99.49% ,RSD =1.5 7% ;精密度RSD为 2 .5 7% ;稳定性RSD为 0 .5 6 % ;回归方程 :Y =132 .5 0 0X - 5 8.2 42 ,线性范围是 1.0 6～ 10 .6 0 μg .结论 该方法操作简便 ,结果准确 ,重现性好 。
Objective To investigate the quality standard method of “Ge Gen Qin Lian”pocket preparation.Methods The qualitative analyses of Kudzuvine root,Large-flowered skullcap root and Chinese goldthread of “Ge Gen Qin Lian” pocket preparation were finished.The reseachers analysed them with TLC method. Then they determined puerarin content of Kudzuvine root by TLC scan.Results The Puerarin content of the pocket was 0.0776%(g/100g);The pick-up recovery was 99.49% and RSD=1.57%;The precision was RSD=2.57% and the stability was RSD=0.56%(within 4h).The regression equation was Y=132.500X-58.242 with correlation coefficient r=0.9960 and the Linear range was 1.06～10.60μg.Conclusions This method was simple,precision and good repeat. It was a quality standard method of “Ge Gen Qin Lian”pocket preparation.
出处
《现代临床医学生物工程学杂志》
2000年第3期190-192,共3页
Journal of Modern Clinical Medical Bioengineering
