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Effect of low-dose X-ray radiation on adaptive response in gastric cancer cell

低剂量X射线诱导胃癌细胞SGC7901适应性反应的实验研究(英文)
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摘要 Objective: We aimed to study the effect and mechanism of low-dose radiation (LDR) on adaptive response of gastric cancer cell. Methods: SGC7901 cells were cultured in vitro, and divided into 4 groups: control group (DO group), low-dose radiation group (D1 group, 75 mGy), high-dose radiation group (D2 group, 2 Gy), low-dose plus high-dose radiation group (D1 + D2 group, 75 mGy + 2 Gy, the interval of low and high-close radiation being 8 h). Cell inhibition rate was detected by cytometry and CCK8 method; the proportion of cell cycle at different times after irradiation was determined by using a flow cytometry. The ATM mRNA levels were detected by using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR). Results: There was no significant different between groups DO and D1, groups D2 and D1 + D2 cell inhibition rate (P 〉 0.05). There was a significant increase G2/M arrest in groups D2 and D1 + D2 than groups DO and D1 after 6 h of radiation and did not recover at 48 h (P 〈 0.05). The ATM mRNA expression of group D2 and D1 + D2 increased highly than that of group DO and D1 (P 〈 0.05). However, differences between group D2 and D1 + D2, group DO and D1 were not statistical significant (P 〉 0.05). Conclusion: LDR cannot induce adaptive response in SGC7901 cells in vitro, which may be associated the regulation of cell cycle, and its ATM mRNA expression cannot be affected by 75 mGy X-ray radiation.
机构地区 Department of Oncology
出处 《The Chinese-German Journal of Clinical Oncology》 CAS 2013年第4期171-174,共4页 中德临床肿瘤学杂志(英文版)
关键词 low-dose radiation SGC7901 cells adaptive response cell cycle X射线辐射 低剂量辐射 适应性反应 胃癌细胞 mRNA表达水平 逆转录聚合酶链反应 细胞周期调控 生长抑制率
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