摘要
目的了解高压电烧伤后大鼠血清CD58含量变化及其意义,探讨乌司他丁(UTI)对其的干预作用。方法将180只成年清洁级SD大鼠按随机数字表法分为对照组、电伤组和治疗组,每组60只。电伤组和治疗组大鼠用调压器及实验变压器制成高压电烧伤模型,对照组接相同装置但不通电致假伤。伤后2min内,对照组和电伤组大鼠经腹腔注射0.9%氯化钠溶液2mL,治疗组大鼠经腹腔注射2×104U/kgUTI注射液2mL。于伤前15min及伤后5min、1h、2h、4h、8h,每时相点采用随机数字表法于各组取10只大鼠,开胸后直视下从心脏采血,分离血清,用ELISA双抗体夹心法检测血清CD58含量。对数据行两因素析因设计的方差分析和LSD检验。结果 (1)对照组和电伤组大鼠血清CD58含量的比较:电伤组CD58含量总体上高于对照组(主效应F=882.901,P<0.01);电伤组CD58含量受伤后时间变量影响(主效应F=81.459,P<0.01),伤后5min～8h各时相均高于本组伤前值(P<0.01),且呈逐渐增高趋势。(2)电伤组和治疗组大鼠血清CD58含量的比较:治疗组CD58含量低于电伤组(主效应F=219.597,P<0.01);治疗组CD58含量受伤后时间变量影响(主效应F=155.774,P<0.01),治疗组伤后5min～8h各时相均高于本组伤前值(P<0.01)。结论高压电烧伤引起大鼠血清CD58含量升高,而UTI能降低高压电烧伤后大鼠血清CD58含量。
Objective To explore the influence of high-voltage electricity on CD58 expression in rats＇ blood serum and the therapeutic effects of Ulinastatin （UTI）. Methods A total of 180 SD rats were randomly divided into three groups, control group, electrical burn group （burn group） and UTI-treated group （treatment group） and each group contained 60 rats. Voltage regulator and transformer were used to make high-voltage electricity burns （HEB） animal model in the burn and treatment groups. The above equipmentwithout electricity was used to treat the rats of the control group. Within 2 minutes after electrical burn, 2 mL of normal saline （NS） and UTI （2×10^4 U/kg） were injected intraperitoneally in control and burn group and in treatment group respectively. The collection of cardiac blood and the preparation of the serum were performed at 15 minutes before HEn, 5 minutes later, 1 hour later, 2 hours later, 4 hours later and 8 hours later （10 rats were selected randomly in above time-point）. The concentration of CD58 was assayed with ELISA. And the experimental data were analyzed statistically with variance analysis and LSD test. Results （ 1 ） The comparison of CD58 expression between the control and burn groups ： the expression in burn group was increased compared with that in control group （ F = 882. 901, P 〈 0. 01 ）, the increased CD58 expression in burn group was in a time-dependent manner and the expression at 5 minutes to 8 hours after the burn was higher than that before the burn （ F = 81. 459, P 〈 0.01 ）. And there was an increased trend for expression of CD58. （2） The comparison of CD58 expression between the burn and treatment groups： the expression in treatment group was reduced compared with that in burn group （ F = 219. 597, P 〈 0.01 ）. The change of CD58 expression in treatment group was linked with time duration and the expression at 5 minutes to 8 hours after the burn was higher than that before the burn （ F = 155. 774, P 〈 0.01 ）. Conclusions CD58 expression was up-regulated in blood serum of rats after HEn and its expression was reduced after UTI treatment.
出处
《中华损伤与修复杂志（电子版）》
CAS
2012年第5期4-7,共4页
Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)
基金
河北省自然科学基金(302551)
关键词
烧伤
电
CD58
乌司他丁
微循环
大鼠
Bums, electricity
CD58
Ulinastatin
Microcirculation
Rats
