摘要
目的 :探讨胆固醇饲养的去卵巢兔接受 17β -雌二醇 (17β -estradiol,E2 )替代治疗后血浆氧化型低密度脂蛋白 (oxidizedLDL ,oxLDL)的变化及其与主动脉粥样硬化形成的关系。方法 :全部兔切除双侧卵巢 ,喂饲含 1 5 %胆固醇的标准颗粒饲粒。 2周后随机分成 4组 ,其中 3组分别用E2 1mg ,2mg和 4mg替代治疗 ,另 1组为对照组。分别在用E2 后 0 ,3 ,8,12周测定血清超氧化物歧化酶 (SOD)活性和oxLDL水平 ,用计算机测定主动脉粥样斑块面积。结果 :17β -雌二醇替代治疗后 ,血清SOD明显上升 (P <0 0 5 ) ,而血浆oxLDL水平和主动脉粥样斑块面积显著降低 (P <0 0 1) ,且与E2 有量效关系。全部实验兔血浆oxLDL水平和主动脉粥样斑块面积呈显著正相关 (r=0 74,P <0 0 1)。结论
AIM: To observe the changes of plasma oxidized low density lipoprotein(oxLDL) levels of ovariectomized cholesterol-fed rabbits with or without 17β-estradiol(E 2) replacement therapy.METHODS: All rabbits were ovariectomized and fed standard chow supplemented with 1.5% cholesterol for 14 weeks. Two weeks after operation, the rabbits were randomly assigned to four groups. Three groups were treated with E 2 1 mg, 2 mg, 4 mg respectively, the other group served as control. Serum superoxide dismutase (SOD) levels and plasma oxLDL levels were measured at 0, 3, 8, 12 weeks after hormone replacement therapy. The aortic atherosclerotic lesion areas were determined by computer. RESULTS: We found that there were striking increase of serum SOD levels ( P<0.05 ) and significant decrease in both the plasma oxLDL levels and the aortic atherosclerotic lesion areas ( P<0.01 respectively). Moreover, a positive correlation was found between plasma oxLDL levels and the areas of atherosclerotic plaque in all rabbits. CONCLUSIONS: Estrogen attenuates atherogenesis in cholesterol-fed ovariectomized rabbits. And this beneficial effect of E 2 may be duo to its lowering of plasma oxLDL level.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2000年第9期814-817,共4页
Chinese Journal of Pathophysiology
基金
湖北省科委"九五"攻关课题基金资助!(No.962P1101)
