摘要
目的建立甘肃礼县掌叶大黄的高效液相色谱指纹图谱,可为科学评价和有效控制其质量提供可靠的方法。方法采用高效液相色谱法,色谱柱为Agela Venusil XBP-C18柱(4.6mm×250mm,5μm);流动相为乙腈-0.1%磷酸溶液,梯度洗脱;流速为1.0mL/min;检测波长为254nm;柱温为40℃。结果掌叶大黄中所含主要成分的色谱峰均得到了有效的分离,在指纹图谱中共有峰有26个,并指认了5个色谱峰。结论该方法对掌叶大黄质量控制以及实现规范化种植提供了参考依据。
OBJECTIVE To establish HPLC fingerprint of Rheum palmatum from Gansu and provide a reliable method for its scientific evaluation and quality control. METHODS The main components were separated on a Agela Venusil XBP-C18 （4. 6 mm× 250 mm, 5 μm） column and detected at 254 nm by gradient elution at the flow rate of 1.0 mL/min using acetonitrile-0. 1% phosphoric acid as the mobile phase, Column temperature was at 40 ℃. RESULTS The main components of thei samples harvested from 8 locations at Li County of Gansu were successfully and effectively separated and 26 peaks could be found from all chromatograms. 5 major peaks were identified based on the comparison with reference substances. CONCLUSION The method can be used for quality control and provide a valuable reference for Good Agriculture Practice of Rheum palmatum.
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2013年第2期187-191,共5页
Journal of Nanjing University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81260622)
