摘要
目的研究Fractalkine(FKN)在狼疮性肾炎(lupus nephritis,LN)患者血液和肾组织中的表达及其意义。方法选取48例LN患者,根据病理分型分为轻型组22例、重型组26例,采用RT-PCR检测患者血液中FKN mRNA的表达、酶联免疫吸附法(ELISA)检测患者血液中FKN蛋白的表达,并与26例正常对照比较;同时通过免疫组织化学染色法测定LN患者肾组织FKN的定位与表达情况。结果与正常对照组相比,LN中轻型组和重型组患者血液中FKN的mRNA和蛋白的表达均明显升高,且重型组升高更为显著(P<0.01),重型组FKN mRNA和蛋白的表达也明显高于轻型组。在LN患者肾组织中,FKN主要表达于肾小球足细胞和肾小管上皮细胞的胞浆中,重型组肾小球FKN阳性细胞数较轻型组明显增多(P<0.01),但皮质间质的FKN阳性细胞数无差异(P>0.05)。结论 FKN在LN患者血液和肾小球中的表达与LN的肾组织病理损伤表现的程度有关。
Objective To investigate the expression of fractalkine（FKN） in the blood and renal tissues of patients with lupus nephritis and explore its significance.Methods According to the pathological classification,48 patients with lupus nephritis were divided into mild group（22 cases） and severe group（26 cases）,with 26 healthy subjects as the control group.RT-PCR and enzyme-linked immunosorbent assay were employed to detect the expression of FKN mRNA and protein in the blood of the subjects,and FKN expression and localization in the renal tissue of the patients with lupus nephritis were detected using immunohistochemical staining.Results The patients in both the mild and severe groups showed significantly increased expression of blood FKN mRNA and protein compared with the normal controls,and the increase was more obvious in severe cases（P0.01）.In the renal tissues of the patients,FKN was located mainly in the cytoplasm of the glomerular podocytes and renal tubular epithelial,and the number of positive glomerular cells number was significantly greater in severe cases than in the mild cases（P0.01）;FKN expression in the cortical interstitium did not show a significant difference between the 3 groups. Conclusion FKN expression in the blood and glomeruli of patients with lupus nephritis is related to the severity of renal pathologies.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2013年第4期520-523,共4页
Journal of Southern Medical University
基金
国家自然科学基金(81160013/H0111)
广西卫生厅中医药科技课题(GZPT1249
GZZC1243)~~
