摘要
目的:探讨不同分化程度人舌鳞状细胞癌组织及细胞系中STAT3及miRNA-21表达的相关性及其和预后的关系。方法:Western Blot法测定舌鳞癌细胞中STAT3表达,免疫组织化学染色法检测舌鳞癌组织标本中STAT3蛋白表达水平,采用荧光实时定量PCR法检测舌鳞癌细胞中miRNA-21的表达,采用荧光原位杂交法测定舌鳞癌标本中miRNA-21表达,Spearman等级相关分析STAT3、miRNA-21表达在舌鳞癌中表达的相关性,Kaplan-Meier法分析STAT3、miRNA-21不同表达状态与生存期的关系。结果:STAT3和miRNA-21在舌鳞癌细胞及组织标本中过表达;舌鳞癌标本中STAT3、miRNA-21随肿瘤分化程度越差,其表达越高;STAT3和miRNA-21表达呈正相关(rs=0.574,P<0.001);STAT3、miRNA-21与生存期为负相关,STAT3和miRNA-21高表达患者的预后较低表达者差。结论:STAT3、miRNA-21的表达状态对预测舌鳞癌患者的预后具有一定的价值。
Objective： This study aims to （i） detect the expression of both microRNA-21 （miR-21） and signal transducer and acti- vator of transcription 3 （STAT3） in oral squamous cell carcinoma （OSCC） tissue samples and cell lines, and （ii） determine the correla- tion between their expression and prognosis. Methods： miR-21 expression in OSCC cell lines and tissue samples was measured by re- al-time polymerase chain reaction and in situ hybridization, respectively. Western blot analysis and immunohistochemical staining were used to determine the STAT3 expression in the OSCC cell lines and tissue samples. The correlations between the miR-21/STAT3 expres- sion and the pathological grade were analyzed using the Spearman rank correlation. Results： Abnormal activations of miR-21 and STAT3 were observed in the OSCC cell lines and the tissue samples, miR-21 （r~=0.879, P=0.000） and STAT3 （rs=0.837,/9=0.000） were positively correlated with the tumor stage classification. Survival rate was higher in the group with miR-21 overexpression than in the group with low expression. Conclusion： miR-21 and STAT3 are potential predictors for the prognosis of OSCC patients. The assess- ment of miR-21 and STAT3 expression can help determine a novel pathway for individualized targeted therapy.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2013年第6期323-327,共5页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金(编号:81172573)
天津市应用基础及前沿技术研究计划(编号:115CYBJC10800)资助~~
