摘要
目的:分析大肠埃希菌的分布特征及耐药性特点,从而为临床提供有效的诊断与治疗依据。方法:采用MicroScan Walk Away96SI全自动细菌鉴定仪分析系统对分离出的310株大肠埃希菌进行鉴定和药物敏感性试验检测其耐药性。并用纸片扩散法表型确证试验检测ESBLs。结果:310株大肠埃希菌分布为宫颈分泌物271株,占87.4%,血培养22株,占7.1%,尿培养10株,占3.2%,其他部位7株占2.3%。20种抗生素中,耐药率前三位的是:氨苄西林(82.5%)、哌拉西林(66.2%)、复方新诺明(50.0%)、其次为环丙沙星(48.7%)、左氧氟沙星(45.3%)、氨苄西林/舒巴坦(41.3%)、头孢曲松(41.2%)、头孢噻肟(41.2%)、头孢他啶(41.2%)、头孢唑啉(41.2%)、氨曲南(41.2%)、头孢吡肟(41.2%)、庆大霉素(40%)、加替沙星(39.9%)、头孢西丁(8.8%)、阿莫西林/克拉维酸(7.5%)、阿米卡星(5.5%)、替卡西林/克拉维酸(3.1%)、而耐药率最低的是:亚胺培南(0)、哌拉西林/他唑巴坦(0)。产ESBLs大肠埃希菌为128株,占41.3%。结论:临床分离大肠埃希菌鉴定结果提示其耐药率和耐药性逐渐增加,因此,在治疗大肠埃希菌感染时,应根据药敏结果及患者的病情合理选用碳青酶烯类、氨基糖苷类等抗生素,可以有效地减少或避免耐药菌株的增加,以提高临床治疗效果及改善患者的病情和预后。
Objective：To study the distribution features and drug resistance of Escherichia coli, so as to provide effective basis for clinical diagnosis and treatment. Methods： Three hundred and ten E. coli strains were identified and carried out drug sensitivity test by MicroScan Walk Away96SI. The K - B method was used to detect ESBLs via phenotype confirmation. Results： The 310 E. coli strains were distributed in cervical secretion （271 strains, 87.4% ）, blood culture（22 strians, 7.1% ）, urine culture（ 10 strians, 3.2% ） and other sites（7 strians, 2.3% ）. They were resistant to 20 kinds of antibiotics, the top three were ampicillin（82.5% ）, piperacillin（66.2% ） and sulfamethoxazole trimethoprim（50.0% ）, while imipenem and piperacillin/tazobactam were not resistant to these E. colis. There were 128 ESBL -producing E. coli strains, accounting for 41.3%. Conclusion： Due to the increasing drug resistance rate of E. coli, it was suggested to select antibiotics in accordance with drug susceptibility test results in E. coli infection treatment, in order to decrease or avoid the drug - resistant strains increase, improving the cura- tive effect, disease condition and prognosis of patients.
出处
《中国卫生检验杂志》
北大核心
2013年第3期767-768,共2页
Chinese Journal of Health Laboratory Technology
关键词
大肠埃希菌
分布特征
耐药特点
Escherichia coli
Clinical distribution
Drug resistance
