摘要
目的建立糖智宁胶囊的定性定量方法。方法采用TLC法对糖智宁方中黄连进行定性鉴别,采用HPLC法测定糖智宁胶囊中葛根素和竹节参皂苷Ⅳa的含量。色谱柱为Kromasil C18柱(4.6 mm×250 mm,5μm);柱温30℃;以0.2%磷酸溶液-乙腈为流动相进行梯度洗脱;流速1 mL min-1;检测波长203 nm。结果薄层色谱鉴别中,特征斑点清晰、分离度好、专属性强、阴性样品无干扰;在HPLC法中,葛根素和竹节参皂苷Ⅳa线性范围分别是:0.122 4～1.836μg(r=0.999 9)、0.249 6～3.744μg(r=0.999 9);平均加样回收率分别为98.3%、97.1%;RSD分别为1.0%、0.7%。结论所建方法专属性强,重复性好,可用于糖智宁胶囊的质量控制。
Objective To establish a qualitative and quantitative research standard of Tangzhining capsules.Methods TLC was employed to identify Coptidis Rhizoma;puerarin and panax japonicas saponins Ⅳ a were determined by HPLC.The chromatograhic separation was Kromasil C18column（4.6 mm×50 mm,5 μm）;and the column temperature was 30 ℃.A linear elution of 0.2% phosphoric acid aqueous solution-acetonitrile was adopted at the flow rate of 1 mL min-1.The UV detection wavelength was 203 nm.Results TLC spots were clear,well-separated and specific without interference of the negative control.Puerarin and panax japonicas saponins Ⅳ a had a good linearity at 0.1224-1.836 μg（r = 0.999 9） and 0.2496-3.744 μg（r = 0.999 9）.The average recoveries were 98.3%（RSD = 1.0%） and 97.1%（RSD = 0.7%）.Conclusion The established standard is suitable for the quality control of TZN capsules.
出处
《中南药学》
CAS
2013年第3期235-237,共3页
Central South Pharmacy
基金
陕西省科技计划项目(编号:2011K16-04-04)
关键词
糖智宁胶囊
定性定量方法
葛根
珠子参
黄连
葛根素
竹节参皂苷Ⅳa
薄层色谱法
高效液相色谱法
Tangzhining capsule
qualitative and quantitative research
radix puerariae
rhizome panacis majoris
Coptis chinensis
puerarin
panax japonicas saponins Ⅳ a
TLC
HPLC
