摘要
[目的]利用红曲霉菌对姜黄素进行微生物转化,并对总转化产物进行初步分离纯化,研究姜黄素微生物转化产物对HepG2细胞LDL受体表达影响。[方法]利用红曲霉菌固体发酵法对姜黄素进行微生物转化,提取的总代谢产物利用硅胶柱层析对产物粗分离后,再通过Real-time PCR和Western免疫印迹法,以姜黄素为阳性对照检测转化产物对HepG2细胞LDL受体mRNA和蛋白表达影响。[结果]与正常对照组相比,5μg.mL-1,10μg.mL-1和15μg.mL-1姜黄素经红曲霉菌转化的产物,都可以显著提高LDLR的表达(P<0.01),并且具有一定的量效关系。[结论]姜黄素转化产物能够通过明显上调HepG2细胞LDLR表达来调节血脂和抗动脉粥样硬化的作用。为我们进一步筛选高效降脂新药奠定基础。
[Aim] In order to research the purified combinational compounds of curcumin transforrnated by M.purureus went of curcumin and its affections on the expression of Low Density Lipoprotein Receptor in HepG2 Cell Line. [Methods] The strain M. purureus went was used for microbial transformation by solid fermentation. Separated the extractive metabolites via Slica column chromatography and set the curcumin as positive control, we study its affections on the expression of raRNA and protein of Low Density Lipoprotein Receptor in HepG2 Cell Line by western-blot and Real-time PCR. [Results] The concentration of 5μg.mL-1,10μg.mL-1 and 15μg.mL-1 of the curcumin metabolites all can significantly promote the expression of Low Density Lipoprotein Receptor,and have certain concentration-response relationship. [Conclusion] Regulating serum lipids and anti-atherosclerosis properties of curcurcumin metabolites transformated by M. purureus went were proved to be enhanced, which could establish the foundation for us to screen new efficient antilipemic drugs.
出处
《浙江中医药大学学报》
CAS
2013年第3期317-320,共4页
Journal of Zhejiang Chinese Medical University
基金
国家自然科学基金(30873366)
浙江省新苗人才计划技术推广(2012R410045)~~
