摘要
目的:建立参桃软肝胶囊的质量控制标准。方法:采用TLC对该制剂中生晒参、当归、丹参、大黄进行定性鉴别,采用HPLC测定人参皂苷Rb1的含量,色谱条件为以乙腈-水为流动相进行线性梯度洗脱,流速1.0 mL·min-1,检测波长203nm。结果:TCL专属性强,均能检出目标物质,且斑点清晰,阴性对照无干扰。人参皂苷Rb1线性范围0.406～4.059 7μg(r=0.999 7),平均加样回收率100.96%,RSD 1.16%。结论:方法简便准确、重复性好,可有效控制参桃软肝胶囊的质量。
Objective：To establish the standard for quality control of Shentao Ruangan capsules.Method：Panax ginseng,Angelica sinensis,Salvia miltiorrhiza,Rheum officinale were identified qualitatively by TCL.The content of ginsenoside Rb1was determined by HPLC,chromatographic conditions were as follows：with acetonitrile-water as mobile phase in gradient elution,the detection wavelength of 203 nm,the flow rate of 1.0 mL·min-1.Result：TCL were reflecting a good specificity and could detect target substance with spots were clear and negative control was without interference.Ginsenoside Rb1 showed a good linearity at the range of 0.406-4.059 7 μg（r=0.999 7）,the average recovery was 100.96% with RSD 1.16%.Conclusion：This established method was simple,accurate and reproducible,which could effectively control the quality of Shentao Ruangan capsules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第8期63-65,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
东莞市高等院校科研机构科技计划项目(2011108101009)
