摘要
目的观察白藜芦醇(resveratrol,Res)对人肝癌细胞系HepG2细胞株增殖及凋亡的影响,探讨Res抑制肝癌生长的可能性。方法对数生长期HepG2细胞根据加入的Res浓度分为4组,分别为对照组(A组)、Res 10μmol/L组(B组)、Res 50μmol/L组(C组)与Res 100μmol/L组(D组),4组均采用MTT法检测细胞生长情况,流式细胞法测定细胞周期,细胞免疫组织化学法检测细胞Bcl-2及Bax蛋白水平。结果 24,48,72h时C,D组细胞增殖抑制率高于B组,D组高于C组(P<0.05);B,C,D组72h时细胞增殖抑制率高于48h时,48h时高于24h时(P<0.05);与A组比较,Res作用24h后B,C,D组S期细胞百分率增加,G2/M期细胞百分率减少(P<0.05),且呈剂量依赖性;与A组比较,Res作用24h后B,C,D组Bax表达增加、Bcl-2表达减少(P<0.05),且呈剂量依赖性。结论 Res能明显抑制HepG2细胞增殖并促进其凋亡,可引起HepG2细胞S期阻滞,可能具有抑制肝癌细胞生长的作用。
Objective To identify the role of resveratrol on the proliferation and apoptosis of HepG2 cells in order to explore the possibility of its inhibition on proliferation initially. Methods HepG2 cells in logarithmic growth phase were divided into group A （control group） , group B （treated with 10 μmol/L resveratrol）, group C （treated with 50 μmol/L resveratrol） and group D （treated with 100 μmol/L resveratrol）. Methyhhiazolyl tetrazolium method was used to detect the cell proliferation inhibition of HepG2 cells. Flow cytometry was used to investigate the effect of resveratrol on proliferation of HepG2 cells. Proteins related to apoptosis were detected with cell immunohistochemistry. Results The proliferation inhibition rate was higher in group C and D than that in group B, and in group D than that in group C in 24, 48 and 72 hours after treatment （P〈0.05）, and was higher in 72 hours than that in 48 hours, and was higher in 48 hours than that in 24 hours after treatment in group B, C and D （P〈0.05）. The S phase rate was significantly higher and G2/ M phase rate was significantly lower in 24 hours after treatment in group B, C and D than that in group A in a dose- dependent manner （P〈0.05）. Bel- 2 protein expression decreased and Bax protein expression increased in group B, C and D in 24 hours after treatment compared with group A in a dose dependent manner （P〈0.05）. Conclusion Resveratrol could enhance apoptosis of HepG2 cells and induce the S phase arrest of HepG2 cells. So it is presumed that resveratrol may suppress proliferation of hepatocellular carcinoma.
出处
《中华实用诊断与治疗杂志》
2013年第4期364-367,共4页
Journal of Chinese Practical Diagnosis and Therapy
基金
河南省科技厅基础与前沿项目(112300410037)
河南省医学科技攻关计划指导性项目(2011030006)
郑州市普通科技攻关项目(121PPTGG492-2)
河南省卫生中青年科技创新人才培养项目(2011年)
关键词
原发性肝癌
白藜芦醇
细胞周期
凋亡
Primary liver cancer
resveratrol
cell cycle
apoptosis
