摘要
采用固相萃取-高效液相色谱-荧光法(HPLC-FLD)测定方便面中苯并(a)芘的含量。根据不同的食品材质对样品提取条件进行优化,采用HiCapt-Benzo苯并(a)芘专用固相萃取柱净化,反相C18(4.6mm×250mm,5μm)色谱柱分离,外标法定量。苯并(a)芘在1.0～200μg/L浓度范围内线性关系良好,相关系数R2=0.9998。本方法的定量限(S/N≥10)在方便面调料中的调味油酱料包为0.5μg/kg,在调味粉料包、菜肉包和面饼都为0.1μg/kg;添加浓度为1.0～20μg/kg时,回收率范围为78.1%～96.7%,相对标准偏差(RSD)0.97%～6.23%(n=6)。该方法前处理简单,分析周期短,具有良好的灵敏度和精密度,适用于方便面中苯并(a)芘的测定。
This thesis developed a HPLC method for Quick determination of the benzo（a） pyrene（BaP） level in the instant noodles with fluorescence detector. These samples were treated by the HiCapt-Benzo solid phase extraction （SPE） column, and then separated by the C18 revered - phase column （4.6mm × 250mm, 5μm ）, quantified with the external standard method. It was showed a good linear relationship at a rang of 1.0-200μg/L, R^2=0.9998. The limit of detection was 0.5μg/kg in the flavored oil and 0.1μg/kg in the noodles. Linear plots were obtained between 1.0μg/L and 200μg/L. The recovery was 78.1%-96.7%, and RSD （n=6） was 0.97%-6.23%. The result showed that the method was sensitive, accurate, convenient and quick ,which was suitable for the B（a） P determination in the instant noodles.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第8期65-67,73,共4页
Science and Technology of Food Industry
关键词
苯并(A)芘
方便面
固相萃取
高效液相色谱
荧光检测
Benzo（a） pyrene
instant noodles
solid phase extraction
high-performance liquid chromatography
fluorescence detector
