摘要
研究超高压压力100～500 MPa处理10 min和400 MPa处理0～15 min对壳青霉杀菌效果的影响,并对处理后的壳青霉进行菌落总数测定、扫描电镜观察和紫外吸收检测,探讨超高压对茶叶中分离出的壳青霉致死效果。结果表明,处理压力和处理时间是超高压致死壳青霉的显著影响因子,且处理压力和处理时间对菌落总数的影响基本符合Logistic回归方程。500 MPa以上的压力处理10 min或者400 MPa处理12 min以上可基本杀灭壳青霉;超高压处理使壳青霉菌丝和孢子细胞壁破裂,胞内物质外溢。
The Penicillium crustosum Thom isolated from Rock tea leaves were subjected to uhra-high pressure （UHP） treatments. The pressures at range of 100 to 500 MPa for 10 min and times at range of 0 to 15 min with 400 MPa were performed in order to investigate the effects of UHP on the mortality of the Penicillium crustosum Thorn. SEM photographs observation and ultraviolet absorbance determination was carried out after treating. Treatment pressure and time showed significant （p 〈0.01） effect of UHP processing on inactivating Penicillium crustosum Thorn. The total counts of Penicillium crustosum Thorn were decreased with the increasing of processing pressure or time, and the decreasing trends were fit to Logistic regression equation. The Penicillium crustosum Thorn were killed above 500 MPa for 10min or 400 MPa for above 12 min. The UHP processing spilt the cell walls of the Penicillium crustosum Thom and its ascospores inducing death with intemal substances leaking out of cells.
出处
《热带作物学报》
CSCD
北大核心
2013年第3期555-559,共5页
Chinese Journal of Tropical Crops
基金
福建省教育厅项目(No.JB11323)
关键词
壳青霉
超高压
压力
时间
细胞超微结构
Penicillium crustosum Thom
Ultra-high pressure
Pressure
Time
Cellular ultra-structure
