摘要
目的探讨液泡型ATP酶C亚基ATP6V0C在不同转移潜能人前列腺癌细胞系中的表达及其意义。方法采用半定量RT-PCR、荧光实时定量RT-PCR和Western blot法检测ATP6V0C在人不同转移潜能前列腺癌细胞系PC-3M-1E8、PC-3M(高转移潜能)和PC-3M-2B4、PC-3(低转移潜能)中的表达。结果 ATP6V0C在PC-3M-1E8、PC-3M细胞系中的mRNA及蛋白表达量均明显高于在PC-3M-2B4、PC-3细胞系中的表达,其中,ATP6V0C在PC-3M-1E8中的表达最高,差异具有统计学意义(P<0.05)。结论 ATP6V0C在高转移潜能人前列腺癌细胞系中的表达明显高于低转移潜能人前列腺癌细胞系,证明其和肿瘤的转移密切相关,有可能成为判断人前列腺癌侵袭和转移的重要指标及治疗前列腺癌的新靶点。
Purpose To investigate the expression and significance of C subunit of vacuolar ATPase （ATP6VOC） in human prostate cancer cell lines with different metastatic potential. Methods The expression of ATP6VOC was evaluated in different prostate cancer cell lines using RT-PCR, real-time RT-PCR and Western blot. Results The expression of ATP6VOC mRNA and protein in PC-3M- 1E8 and PC-3M cell line with high metastatic potentiality was significantly higher than that in PC-3M-2B4 and PC-3 cell line with low metastatic potential. Of them all, the expression of ATP6VOC in PC-3M-1E8 was highest. Conclusion The expression of ATP6VOC in PC-3M-1 E8 and PC-3M cell line with high metastatic potential is significantly higher than that in PC-3M-2B4 and PC-3 cell line with low metastatic potential, suggesting that ATP6VOC is closely related to prostate cancer metastasis. Therefore, ATP6VOC is an impor- tant prognostic indicator in judgement of prostate cancer cell growth, progression and metastasis, and targeting ATP6VOC may be a promising strategy against human prostate cancer.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2013年第4期385-388,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金面上项目(30971142)
