摘要
目的:探讨VEGF-C反义核酸对结直肠癌LoVo细胞体内生长的影响。方法:20只裸鼠随机均分为实验组与对照组,实验组接种转染反义VEGF-C核酸的LoVo细胞,而对照组接种转染空白质粒的LoVo细胞。观察两组肿瘤的生长情况,21 d后处死动物取移植瘤标本,用免疫组化法检测移植瘤组织中的微淋巴管密度(MLD)和微血管密度(MVD)。结果:两组的成瘤率均为100%;实验组与对照组接种14 d后的肿瘤体积分别为(382.0±152.8)mm3和(454.2±148.7)mm3,21 d后为(745.0±250.9)mm3和(1 574.4±506.2)mm3,差异均有统计学意义(均P<0.05);实验组肿瘤组织中MLD与MVD计数均较对照组明显减少([11.75±2.22)/0.72 mm2vs.(28.50±2.65)/0.72mm2,(47.75±2.99)/0.72 mm2vs.(53.73±3.50)/0.72 mm2](均P<0.05)。结论:转染VEGF-C反义核酸可抑制结直肠癌LoVo细胞移植瘤在裸鼠的体内生长,并抑制移植瘤淋巴管与血管的生成。
cells. ： To investigate the effects ofVEGF-C antisense RNA on the in vivo of colorectal carcinoma LoVo Methods： Twenty nude mice were equally randomized into experimental group and control group. Mice in experimental group were inoculated with anti-sense VEGF-C transfected LoVo cells, and those in control group were inoculated with empty plasmid transfected LoVo cells, qqae growth of the tumor xenografts in mice was observed.Finally, mice were sacrificed at 21 d after inoculation, and the microlymphatic density （MLD） and microvessel density （MVD） in tumor xenograft tissues were measured through immunohistochemical staining. Results： There was no difference in tumor formation rates between the two groups （both were 100%）. The volumes of the implanted tumor in experimental group and control group were （382.0±152.8） mm^3 and （454.2±148.7） mm^3 at 14 d post-inoculation, and were （745.0±250.9） mm^3 and （1 574.4±506.2） mm^3 at 21 d post-inoculation respectively, and the differences between them had statistical significance （both P〈0.05）. Both MLD and MVD in the tumor tissues from experimental group were significantly lower than those from control group [（11.75±2.22）/0.72 mm^2 vs. （28.50±2.65）/0.72mm^2, （47.75±2.99）/0.72 mm^2 vs. （53.73±3.50）/0.72 mm^2] （both P〈0.05）. Conclusion： The growth of colorectal carcinoma LoVo cell xenografts in nude mice can be suppressed by VEGF-C antisense RNA transfection and that may also inhibit the lymphangiogenesis and angiogenesis in tumor xenografts.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2013年第4期452-455,共4页
China Journal of General Surgery
