摘要
目的探讨白藜芦醇(Res)对糖尿病心肌病大鼠心肌损伤的保护作用及机制。方法采用链脲佐菌素(STZ)诱导2型糖尿病大鼠模型,给予不同剂量Res(10,20,40 mg/(kg.d))治疗6周后,检测各组大鼠空腹血糖、心脏质量指数、左心室质量指数、血清及心肌超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量;Masson染色观察胶原表达变化及测量心肌胶原容积百分数;比较各组大鼠心肌中总抗氧化能力(T-AOC)及过氧化氢(H2O2)的含量,Western bolt检测心肌组织中p22phox蛋白表达。结果与正常组相比,模型组大鼠空腹血糖、心脏质量指数及左心室质量指数明显增高,心肌胶原容积分数明显升高,血清及心肌SOD活力及心肌T-AOC明显降低,心肌组织p22phox蛋白表达及H2O2和MDA含量显著升高(P均<0.01)。与糖尿病组相比,Res各剂量组灌胃6周后上述指标均得到明显改善(P<0.05或0.01)。结论 Res可下调2型糖尿病大鼠心肌p22phox蛋白过度表达,减轻氧化应激对心肌的损害,发挥心肌保护作用。
Objective It is to investigate the protective effects and mechanism of Resveratrol on myocardial injury in diabetic rats. Methods Streptozotoein-induced diabetic rats were given Resveratrol （10, 20, 40 mg/kg/d） for 6 weeks. The changes in blood glucose （BG） , cardiac mass index and left ventricular mass index were calculated, and serum and myocardium superoxide dismutase （SOD） and malondialdehyde （MDA） were determined. Morphological change was observed by Masson staining and myocardial collagen volume fraction （CVF） was measured by image analyzer. The total antioxidant capacity （T -AOC） and hydrogen peroxide （H2 O2 ） in myocardial tissue were compared. In addition, the expressions of p22phox protein in myocardium were determined by western bolt. Results Compared with the normal control group, the BG, cardiac mass index, left ventricular mass index and CVF values were significantly increased. The activities of SOD and T - AOC were significantly reduced. The expression of p22phox protein and the content of MDA and H202 were also significantly increased （P 〈 0.01 ）. Compared with the diabetic group, all above indexes of Resveratrol treatment groups had been improved by gavage 6 weeks （P 〈 0.05 or 0.01 ）. Conclusion Resveratrol can down-regulate the over-expression of myocardial p22phox protein in type 2 diabetic rats, relieve the myocardial damage induced by oxidative stress, thus plays a protective role in the myoeardium of diabetic rats.
出处
《现代中西医结合杂志》
CAS
2013年第14期1498-1501,共4页
Modern Journal of Integrated Traditional Chinese and Western Medicine
