摘要
目的观察银杏提取物(EGB)对大鼠肝缺血再灌注损伤(I/R)时CO、ET含量变化及对细胞凋亡相关蛋白Bcl-2的影响。方法建立大鼠肝缺血再灌流模型,24只大鼠随机分成三组:假手术组(Sham组)、I/R组和I/R+EGB组。观察大鼠肝组织形态学改变,测定血浆中一氧化碳(CO)、内皮素(ET)含量变化、并测定凋亡相关蛋白Bcl-2的变化。结果 HE染色显示EGB组肝损伤明显减轻;EGB组肝血浆中CO含量升高、ET含量降低,与Sham组及I/R组相比有显著差异。EGB组凋亡相关蛋白Bcl-2明显高于I/R组。结论 EGB对大鼠肝I/R损伤具有保护作用,其机制可能与增强内源性CO表达、降低ET含量,及增加细胞相关蛋白Bcl-2表达有关。
Objective To study the effect of ginkgo biloba extraction on the change of CO, ET and apoptosis protein Bcl-2 in hepatic ischemia-reperfusion injury of rat model. Methods The hepatic ischemia-reperfusion injury model was established. A total of 24 rats were randomly divided into 3 groups： the Sham group, I/R group and I/R ＋ EGB group. Changes of tectology were observed, and the changes of CO, ET content, as well as apoptosis protein Bcl-2 were also measured. Results In the EGB group, the tissue content of CO and cell apoptosis protein Bcl-2 was obviously higher than that in the I/R group; also, the ET in the EGB group was significantly lower compared to that in the I/R group. Conclusions EGB can help to protect I/R injury and suppress the apoptosis of hepatic tissue cell during hepatic ischemia/reperfusion injury. It might attribute to its effect on improving the content of CO, impressing the content of ET, and increasing the expression of cell apoptosis protein Bcl-2.
出处
《临床医学工程》
2013年第4期415-416,共2页
Clinical Medicine & Engineering
