摘要
采用邻苯三酚自氧化法、硫代巴比妥酸比色法、DMPD法、甲基紫法和SDS-PAGE法,检测了NaHS对超氧阴离子的清除能力、脂质过氧化的抑制作用、DMPD.+自由基的清除能力、清除羟自由基的能力及蛋白质氧化降解的保护作用.研究结果表明,NaHS在各体系中均具有很强的作用,且呈浓度依赖关系.脂质过氧化的半抑制浓度为22.04μg/mL,对超氧阴离子、DMPD和羟自由基的半清除率分别为3.44、1.26和54.07μg/mL,活性均远高于人工合成抗氧化剂Trolox.对蛋白质的氧化损伤在2μg/mL时已有明显的保护作用,作用也比Trolox强.这些结果表明NaHS在这些反应系统中均表现出突出的抗氧化活性.
The antioxidant activities of NariS were evaluated by vary methods to meas- ure the scavenging effects of superoxide radical, hydroxyl radical and DPMP＂ ＋ radical, the protection against of rat liver microsome lipid peroxidation and protein oxidative damage. The results showed Naris exhibited high antioxidant activities in all these systems and the functions are in concentration-dependent manner. The IC50 in lipid peroxidation is only 22.04 μg/mL. The SC50 to superoxide radical, DPMP＂ ＋ and hydroxyl radical are 3.44,1.26,and 54.07 μg/mL, respectively. All these actions are stronger than Trolox-reformed Vitamin E. The protection to protein oxidative damage is effective even in 2 μg/mL and stronger than Trolox, too. These results showed Naris owed excellent antioxidative effects in all these systems.
出处
《华中师范大学学报（自然科学版）》
CAS
北大核心
2013年第2期242-245,共4页
Journal of Central China Normal University：Natural Sciences
基金
国家自然科学基金项目(30960464)
教育部留学回国人员科研启动基金项目(2011)
