摘要
目的探讨表达于SiSo细胞系上的受体结合癌抗原(RCAS1)在肾透明细胞癌(RCC)中的表达及临床意义。方法应用免疫组织化学法检测RCAS1在62例RCC组织及14例癌旁肾组织中的表达,用Western blot分别检测了3例肾透明细胞癌和3例癌旁肾组织中RCAS1的蛋白表达,并分析了RCAS1蛋白的表达与临床病理特征的关系。结果免疫组织化学检查RCC组织中RCAS1表达率为79.0%(49/62),与癌旁组织表达率14.3%(2/14)比较,差异有显著性(P<0.01)。RCAS1在RCC的表达随着病理分级降低而增高(P<0.05),与RCC的病理分化程度呈负相关(P<0.01),与RCC的临床分期呈正相关(P<0.01)。Western blot检测RCAS1蛋白结果显示,RCC组织中的RCAS1表达明显高于癌旁组织(P<0.01)。结论 RCAS1在RCC中高表达,且与其病理分级、临床分期相关,检测RCAS1的表达有助于对RCC的病理分级、临床分期和预后的判断。
Objective To investigate the expression of receptor-binding cancer antigen expressed on SiSo cells （RCAS1） in renal cell carcinoma （RCC） and to study their significance in the progression and prognosis of the RCC. Methods Expression of RCAS1 was detected by immunohistochemistry technique in 62 cases of RCC and 14 cases of pare-cancerous renal tissue. The expression of RCASl was also investigated by western blot in 6 cases with renal tissues （3 cases of RCC and 3 cases of pare-cancerous tissue）. The relationship between expression of RCAS1 and clinical pathological features was analyzed. Results The ratio of RCASl protein expression was increased in RCC tissues （79. 0% ） compared with pare-cancerous tissue （ 14. 3% , P 〈 0. 01 ） using streptavidin-biotin pemxidase （S-P） method. Within the specimens of RCC group, the differences were statistically significant among WHO pathological grade and clinical stage （P 〈0. 05）. There was a negative correlation between the RCAS1 expression and pathological grades （P 〈 0. 01 ）, however,there was a positive correlation between the expression of RCAS1 and clinical grades （P 〈 0. 01 ）. The expression of RCAS1 in RCC tissues assessed by Western blot was high than pare - cancerous tissues （ P 〈 0. 01 ）. Conclusion High expression of RCAS1 in RCC tissues was related to the tumor clinical stage and tumor pathological grade. Detection of RCAS1 expression in RCC was useful to study the pathological grade, clinical stage and prognosis.
出处
《中南医学科学杂志》
CAS
2013年第2期131-134,139,共5页
Medical Science Journal of Central South China
基金
衡阳市科学技术发展计划(2011KJ49)
