摘要
本实验用Nissl染色法、Bielschowsky Gros Lawrentjew染色法、常规透射电镜、行为活动测定、双侧海马微量给药、海马神经元原代培养、活细胞连续照相、全细胞膜片钳记录、细胞内游离Ca2 +浓度测定及P5 3蛋白免疫组化测定等方法 ,观察了睫状神经营养因子 (CNTF)对应激引起动物行为变化和海马神经元形态学变化的影响 ,探讨了CNTF的部分作用机制。结果表明 ,急性应激不引起大鼠海马神经元损伤 ,CNTF不能改善急性应激时的焦虑样行为。但CNTF可显著减轻慢性应激引起的大鼠海马神经元损伤 ,并改善慢性应激时的抑郁样行为。CNTF可能通过早期快速抑制谷氨酸 (Glu)膜电流、胞内游离钙 ( [Ca2 +]i)的升高 ,以及后期核内效应 ,发挥其保护神经元的作用 。
Effect of ciliary neurotrophic factor (CNTF) on behavior and morphology of hippocampal neurons were observed and its mechanisms in rats were explored by Nissl staining, Bielschowsky Gros Lawrentjew staining, transmission electron microscopy, behavior determination, primary culture of hippocampal neuron, running photography of living cell, whole cell patch clamp recording, detection of intracellular free Ca 2+ and immunohistochemical detection of P53 protein. The results showed that there was no statistically significant change in the morphology of hippocampal neurons as a result of acute stress. The behavioral activity was increased during acute stress stage, which was [CM(36]not affected by CNTF. In chronic stress stage, neuronal damage in hippocampus was significant, and behavioral activity was significantly decreased under basal line. Administration of CNTF into bilateral hippocampus prevented neurons from damage and improved behavior. In vitro, CNTF could significantly suppress channel current, intracellular Ca 2+ content and the expression of P53 protein in the nucleus induced by glutamate. The results suggested that the protective effect of CNTF may involve rapid effects on cell membrane and cytoplasma, and delayed effects on nucleus, thereby improve behavioral defects.
出处
《生理科学进展》
CAS
CSCD
北大核心
2000年第2期131-134,共4页
Progress in Physiological Sciences
基金
国家自然科学基金 !(396 70 2 59)
军队医药卫生科研基金 !(96M 0 77
98M 0 79)
关键词
应激
睫状神经营养因子
海马神经元
形态学
Stress
Ciliary neurotrophic factor
Hippocampal neuron
Morphology
Signal transduction
