摘要
采用光镜与电镜结合 ,对角蟾属 16个种 18个居群精子的形态结构进行观察和比较 ,并通过计算机图像处理对其各部位长度进行测量和计算 .结果表明 :角蟾属的精子主要包括头和尾两部分 ,头部呈螺旋状 ,尾部为波状或弯曲状 .根据精子的头部形状和量度 ,角蟾属的精子可分为 2类 .角蟾属内种 (或亚种 )间精子存在一定的差别 ,但同种不同地理居群和不同个体的精子形态相同 ,仅长度略有变化 .本文结果与形态学研究结果基本相吻合 ,可为该属物种的分类提供新的佐证 .图 1表 1参
The shapes of spermatozoa of 16 species of genus Megophrys from China,including Megophrys zhangi,M.kuatunensis,M.boettgeri,M.parva,M.glandulosa,M.medogensis,M.omeimontis,M.spinata,M.jingdongensis,M.wushanensis,M.wuliangshanensis,M.minor.brachykolos,M.m.binchuanensis,M.m.minor,M.gigantica and M.shapingensis ,were examined with light and transmission electron microscopy and the sizes of spermatozoa were measured on enlarged light photomicrographs using computer image analysis.The results show that in Megophrys the spermatozoa are thin and long [90.52～186.71(123.15±26.66) μ m] and consist of two parts, head and tail. The head is spiral and corkscrew shaped, and the shape of tail is winding.The spermatozoa can be divided into two groups based on the measurements of head. The spermatozoa of group Ⅰ are short (116.45±5.06 μ m) and those group Ⅱ are long(176.77±4.69 μ m).The spermatozoa shape of Megophrys differs from that of the other families of Anura.The differences in morphological characteristics of spermatozoa also exist among the species (subspecies)of Megophrys , but the spermatozoa shape is very similar among different individuals and populations in the same species. Spermatozoa vary slightly in length. The result from this study coincides in that from morphological studies and may be a useful method for classification of Megophrys . Fig 1, Tab 1, Ref 11
出处
《应用与环境生物学报》
CAS
CSCD
2000年第2期161-165,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金重点项目!(No:39720080 )
中国科学院分类区系特别支持项目
中国科学院西南基地项目
中国科学院成都地奥科学基
关键词
两栖纲
锄足蟾科
角蟾属
精子形态
Amphibia
Pelobatidae
Megophrys
morphology of spermatozoa
