摘要
利用２０个随机引物对厚皮甜瓜８个亲本与４个杂交种进行ＲＡＰＤ分析。１０个引物的扩增ＤＮＡ谱带具有多态性，亲本具有各自的特异谱带，绝大部分Ｆ１的扩增带型是双亲的互补带。各试材的相似系数范围为６３．６％－９６．５％。遗传距离聚类分析图表明，Ｆ１易与双亲或其一聚在一起。上述结果说明，可以利用ＲＡＰＤ标记在ＤＮＡ水平上进行厚皮甜瓜的亲缘关系及纯度的鉴定。
20 random primers, 8 parental varieties and their 4 F1 of Hami-melon (Rock melon) (Cucumis subspecies.melo pang. )were analysed by RAPD technique.The amplified products of 10 primers showed polymorphism.All of the parental varieties had their specific bands while most of the F1 showed mutually supplementary hands of their parents.The range of similarity coefficients were from 63.6 to 96.5%. UPGMA cluster on the basis of genetic distance indicated that F1 were clustered with their parents easily. The results suggested that the kinship and purity of Hami -- melon materials could be appraised using RAPD analysis on DNA lever.
出处
《果树科学》
CSCD
北大核心
2000年第4期295-299,共5页
Journal of Fruit Science
