摘要
目的探讨 CD_(15) mRNA 及其蛋白表达与胃癌病理生物学行为的关系及 CD_(15) mRNA 表达与其蛋白表达之间的相关性,为观测胃癌侵袭转移潜能和评估胃癌患者预后寻求一个新的客观生物学指标。方法应用原位杂交及高敏感性 CSA 免疫组化方法和 CD_15s特异性 mAb,对于手术切除的17例早期胃癌,21例中期胃癌和57例晚期胃癌组织进行 CD_15mRNA 及其蛋白检测,并结合肿瘤的病理生物学行为和临床随访资料进行分析。结果在原发性胃癌中,CD_(15) mRNA 及其蛋白的表达阳性率分别为89.5%(85/95)和86.3%(82/95).CD_(15)mRNA 及其蛋白表达阳性率均在晚期胃癌96.5%(55/57),93.O%(53/57)明显高于早期胃癌76.5%(13/17),70.6%(12/17)和中期胃癌81.0%(17/21),P<0.05.CD_(15) mRNA 表达与蛋白表达水平具有一致性,均与胃癌浆膜浸润,淋巴结转移和患者预后呈正相关(P<0.05).15例原发和淋巴结转移性胃癌组织均呈CD_(15) mRNA 及其蛋白阳性表达。结论 CD_(15) mRNA 及其蛋白异常表达与胃癌的临床病理生物学行为密切相关,特别是与胃癌细胞的转移潜能和胃癌患者的不良预后密切相关,CD_(15)蛋白水平的表达可以间接反映其mRNA 转录水平,并可作为是预测胃癌转移潜能和评估胃癌患者预后的一个新的生物学指标。
To study the relationship between CD_(15) mRNA and its protein expression and pathobiological behavior of gastric carcinoma,and provide a new objective biological index to evaluate the invasive potential and prognosis of gastric carcinoma. METHODS In situ hybridization and high sensitive CSA immunohistochemical method were used to detect the expression of CD_(15) mRNA and its protein in 17 samples of early stage gastric carcinoma,21 of metaphaee gastric carcinoma,and 57 of advanced gastric carcinoma,all of them were obtained by operative excision.The study was also combined with analysis of the pathobiological behavior and clinical foUow-up. RESULTS The positive rate of CD_(15) mRNA and protein were 89.5% and 86.3% in primary gastric carcinoma, respectively.The positive expressions were all presented in advanced gastric carcinoma,the rates being 96.5% and 93.0%,respectively,which were obviously higher than that in the early stage(76.5% and 70.6%),and metephase gastrocarcinoma(81.0%,P<0.05).The expression of CD_(15) mRNA was consistent with that of its protein,and both of them were positively correlated with serosa infiltration,lyphmnode metastasis and the prognosis of gastric carcinoma(P<0.05).Positive expression of CD_(15) mRNA and protein was presented in 15 cases of primary gastric carcinoma and gastric carcinoma with lymphnode metastasis. CONCLUSION The abnormal expression of CD_(15) mRNA and protein was associated with the pathobiological behavior of gastric carcinoma,especially with the metastatic potential of the tumor and bad prognosis of the patients, The expression level of CD_(15) mRNA and protein could be a new biological index to predict the metastatic potential of gastric carcinoma and evaluate the prognosis of patients.
出处
《世界华人消化杂志》
CAS
2000年第8期851-854,共4页
World Chinese Journal of Digestology
关键词
胃肿瘤
肿瘤转移
预后
CD15蛋白
原位杂交
Subject headings stomach neoplasms
neoplasm metastasis
prognosis
CO_(15)protein
in situ hybridization
immunohistochemistry
