摘要
目的探讨小鼠骨髓间充质干细胞（BMSC）各亚群在心肌修复中的作用。方法小鼠心脏干细胞表面分化抗原CD45、CD31检测BMSC后，以其阴性及阳性为标准分选得到4个细胞亚群，分别为SCA-1＋／CD45’／CD31＋、SCA-1＋／CD45＋／CD31、SCA-1＋／CD45／CD31-和SCA-1＋／CD45-／CD31＋亚群。将4个细胞亚群及未分选群分别经尾静脉注入心肌梗死小鼠体内，于注射后48h完成心脏彩超心功能测定及小动物活体成像检查，并行心脏梗死原位c—kil（心肌干细胞标志抗原之一）免疫组织化学、苏木素一伊红染色及原位免疫组织化学心肌特异抗原检测。结果心脏彩超提示SCA-1＋／CD45＋／CD31＋细胞亚群注入心肌梗死小鼠体内后48h，其左心室收缩末期直径和舒张末期直径注入前后的差值均明显小于注入其余细胞亚群及未分选细胞群的小鼠（P均〈0．05），而左心室射血分数、短轴缩短率注入前后的差值则均高于注入其余细胞亚群及未分选细胞群的小鼠（P均〈0．05）。小动物活体成像显示SCA-1＋／CD45＋／CD31＋细胞亚群注入心肌梗死小鼠体内后48h，其荧光强度高于注入其余各细胞亚群及未分选群的小鼠（P均〈0．05）。各细胞亚群及未分选群干细胞注入心肌梗死小鼠后48h心肌局部均可见心肌干细胞大量增加。各细胞亚群动员心肌干细胞的能力为SCA-1＋／CIM5＋／CD31＋细胞亚群〉SCA-1＋／CD45-／CD31＋细胞亚群〉SCA-1＋／CD45-／CD31-细胞亚群〉SCA-1＋／CD45＋／CD31-细胞亚群。SCA-1＋／CD45＋／CD31＋细胞亚群注入心肌梗死小鼠后48h，梗死心肌局部可见其向心肌样细胞分化。结论BMSCSCA-1＋／CD45＋／CD31＋细胞亚群可改善心肌梗死小鼠心功能，其抗凋亡及动员自身心肌干细胞的能力优于其他细胞亚群及未分选群，并可分化为心肌样细胞。
Objective To search for the bone mesenchymal stem cell （MSC） subgroup which might be more effective on repairing myocardial damage. Methods In this experiment, four MSC subgroups were defined based on the surface differentiation antigen detection of mouse bone mesenchymal stem cells （mBMSCs） ： SCA-1 ＋/CD45 ＋/CD31 ＋, SCA-1 ＋/CD45 ＋/CD31 - , SCA-1＋/CD45 -/CD31 - and SCA-1 ＋ / CD45-/CD31 ＋. These subgroup ceils and unselected mBMSCs were injected into infarcted mouse via tail vein. Echocardiographic heart function measurement and in vivo DiR-labeled stem ceils imaging were performed at 48 h after injection. In situ C-kit （ a flag antigen of cardiac stem cells） and cardiac-specific differentiation antigen immunohistochemistry detection was made in the infarcted myocardium. Results The capacity of the SCA-1 ＋/CD45 ＋/CD31 ~ cells on improving heart function was significantly higher than other cell groups（ all P 〈 0. 05 ）. In vivo imaging showed that the mean fluorescence intensity of the SCA-1 ＋/ CD45 ＋/CD31 ＋ cells was also higher than other cell groups （all P 〈 0. 05 ）. Number of cardiac stem cells in the infracted myocardium was significantly increased after the injection of all subgroup cells and unsorted mBMSCs ceils for 48 h compared untreated infracted myocardium. The capacity of mobilizing cardiac stem ceils is as follows ： SCA-1＋/CD45 ＋/CD31 ＋ 〉 SCA-1 ＋/CD45 -/CD31 ＋ 〉 SCA-1＋/CD45 -/CD31 - 〉 SCA- 1 ＋/CD45＋/CD31 - . Conclusion The SCA-1 ＋/CD45 ＋/CD31 ＋ subgroups of mBMSCs exhibites the highest capacity to improve cardiac function after myocardial infarction and to mobilize autologous cardiac stem cells compared with other mBMSCs subgroups and unsorted mBMSCs cells.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
2013年第3期210-214,共5页
Chinese Journal of Cardiology
基金
国家临床重点专科建设项目（G20120233）
江苏省医学重点学科（XK201118）
江苏省普通高校研究生科研创新计划资助项目（CXLX12_0841）
苏州大学优秀博士论文立项项目（SD2012016）
关键词
心肌梗死
间质干细胞移植
Myocardial infarction
Mesenchymal stem cell transplantation
