摘要
分析正常肺、支气管组织和肺癌组织的激光诱发自体荧光 (LIAF)光谱特征 ,探讨利用LIAF区分正常肺、支气管组织与肺癌组织的可行性。方法 收集肺癌手术标本 42例 ,每一标本的不同部位即肿瘤组织和正常肺组织及正常支气管组织 ,分别平均测定 4～ 5条光谱曲线 ,共测得 196个部位的LIF光谱图 ,其中癌组织 74个 ,正常肺组织 6 7个 ,正常支气管组织 5 5个。使用三倍频YAG激光(波长 35 5nm)和光学多道分析仪 (OMA)测定各部位的LIAF光谱 ,读出每条光谱曲线的主峰波长 ,峰值相对强度 ,5 80nm和 6 0 0nm处的荧光强度及其比值 (I580nm/I60 0nm)。结果 (1)正常肺、支气管组织和肺癌组织LIAF光谱的主峰波长 (nm)差别不大 ,分别为 471 6± 5 3、46 1 9± 4 3和 46 3 6± 3 6。(2 )肺癌组织的峰值相对强度 (2 42 2 7± 434 9)大于正常肺LIAF光谱组织 (1485 4± 46 2 1) ;而支气管的峰值强度最高 (5 1376±8332 )。 (3)正常肺组织在主峰右侧 5 6 0nm及 6 0 0nm处有两个次峰 ,正常支气管组织在 5 80nm与 6 0 0nm表现为一小坪 ,而肺癌组织主峰右侧光谱曲线平滑下降 ;I580nm/I60 0nm比值在正常肺组织为 0 831± 0 177,正常支气管组织为 1 0 41± 0 117,肺癌为1 2 6 9± 0 147(P <0 0 0 1)。结论 正常肺。
Objective To approach the regularity of laser-induced autofluorescence (LIAF) of normal lung and lung cancer and explore the feasibility of spectroscopy in the differentiation between normal lung tissue, normal bronchial tissues and lung cancer. Methods The LIAF spectra of normal lung and lung cancer in 42 surgical specimens were examined with a detecting system which consists of an YAG laser(wavelength 355 nm) and an optical multichannel analyzer(OMA), pathological examinations were done in every testing spots. Results (1)The location of the principle spectral peaks of the normal lung and lung cancer are different, there is a red shift in the peak emission wavelength in the normal lung tissue (471 6 nm±5 2 nm) than that of lung cancer(461 9 nm±4 3 nm) and bronchus(463 6 nm±3 6 nm). (2)The relative fluorescence intensity of the lung cancer(24 227±4 349)is higher than that of the normal lung(14 854±4 621), but the bronchus is the highest(51 376±8 332). (3)In the normal lung tissue spectrum, there are two subsidiary maxima located clearly at 560 nm and 600 nm. The cancer tissue spectrum shows an almost structureless and smoother profile with no subsidiary maxima. The mean relative fluorescence intensity ratio values at 560 nm and 600 nm were 0 831±0 178 for normal lung tissue, 1 041±0 117 for normal bronchus and 1 269±0 147 for lung cancer(P<0 001). Conclusions There is a significant difference between the autofluorescence spectra of normal lung, normal bronchus and lung cancer, and it is feasible to differentiate lung cancer from normal pulmonary and bronchial tissues using LIAF spectroscopy.
出处
《中国激光医学杂志》
CAS
CSCD
2000年第3期137-140,共4页
Chinese Journal of Laser Medicine & Surgery
关键词
肺肿瘤
激光
荧光
光谱分析
Lung neoplasms
Lasers
Fluorescence
Spectrum analysis
