摘要
以抗麦长管蚜小麦种质资源98-10-35和敏感种质资源1376为亲本,以其杂交F1、F2、F3、BC1和BC2群体为研究对象,采用遗传模型检验、配合力验证和基因频率及基因型频率对其进行分析,结果表明:回交群体BC1是单一正态分布,BC2是2个正态分布且所占比例符合1∶1的分离比,推测主基因表现为完全显性;其基因频率和基因型频率分别为:P(A)=46.7%,P(a)=53.3%,P(AA)=21.8%,P(Aa)=49.8%,P(aa)=28.4%;分析其配合力可知,一般配合力为0.006 16,特殊配合力为-0.220 41;同时由田间表型鉴定结果可知,亲本98-10-35和F1均表现为抗蚜,F2代群体经x2测验抗感分离为3∶1(x2=1.667,P=0.168),符合孟德尔理论的比例。由此分析可知,小麦种质资源98-10-35对1376的抗蚜性是由1对显性基因控制。通过对种质资源抗蚜世代遗传变异规律的分析,以期为抗麦长管蚜新品种选育提供理论依据和抗原材料,进一步为小麦抗蚜育种提供理论支持。
In the present study, the synthetic wheat line 98-10-35 is resistant and the synthetic wheat line 1376 is susceptible to S. avenae in greenhouse and field tests. Several related populations including F1 , F2, F3 and BC1 bred from 98-10-35/1376 are analyzed by genetic model,combining ability, gene frequency and genotypic frequency. Results indicated that： backcross population of BC1 was a single normal distribution, BC2 ,two nor- mal distribution, then the proportion of the 1 ： 1 segregation ratio, the performance of a single dominant gene. The gene and genotype frequencies were ： P （A） = 46.7%, P （a） = 53.3%, P （AA） = 21.8%, P （Aa） = 49.8% , P（aa） = 28.4%. The combining ability analysis showed that the general combining ability 0. 006 16, specific combining ability -0. 220 41 ;The phenotypic identification results from the field showed that 98-10- 35 and F1 were resistance to aphid ,and the resistance analysis of their F2 segregation populations showed that both segregation ratios were corresponding with the expected Mendelian segregation ratio of 3 ： 1 by Chi-square value （x2 = 1. 667 ,P = 0. 168 ）suggested that the aphid resistance in wheat lines 98-10-35 to 1376 was con- trolled by a single dominant gene respectively. In work for the wheat aphid resistance breeding, we order to provide theoretical support and guidance for practical analyzed genetic variation in anti-aphid generations of germplasm resources.
出处
《种子》
CSCD
北大核心
2013年第4期5-9,共5页
Seed
基金
河南科技大学人才基金(编号:09001595)
河南省教育厅自然科学研究基金(编号:2011 A 180011)共同资助
