摘要
采用离子交换柱层析分离纯化得到真姬菇多糖HMP-1。HMP-1总糖含量为99%,经高效分子排阻色谱(HPSEC)分析,证明HMP-1为单一组分。单糖分析结果表明,HMP-1由葡萄糖和半乳糖组成,含量分别为67.36 mg/g和33.36 mg/g。采用高效凝胶尺寸排阻色谱-十八角激光散色仪-示差检测仪-紫外检测器联用(HPSEC-MALLS-RI-UV)技术,测得HMP-1的平均分子质量(Mw)为4781±0.6 ku,分子回旋半径值(Rg)为(17.1±1.6)nm。甲基化分析确定HMP-1主链由(1,4)葡糖糖组成,含有(1,6)半乳糖的分支。体外实验表明,HMP-1虽然对人胃腺癌细胞(AGS)、结肠腺癌细胞(DLD-1)、子宫颈癌细胞(HeLa)没有明显的抑制作用,但可以促进小鼠巨噬细胞Raw264.7的增值和剂量依赖性地提高巨噬细胞中NO的生成量,具有免疫调节活性。
HMP-1 was isolated from Hypsizigus marmoreus fruit body and fractionated by ion-exchange chromatog- raphy. The carbohydrate content of HMP-1 was 99%. HMP-1 was proved to be homogeneous by high performance size exclusion chromatography （HPSEC）. Their monosaccharide levels were significantly different, including the glu- cose content （67.36mg/g） and galactose content （33.36mg/g）. The average molecular weight （Mw） of HMP-1 was （4 781 _＋ 0.6）kDa and its radius of gyration （Rg） was （17.1 ＋ 1.6）nm determined by HPSEC-MALLS-RI system. The backbone of HMP-1 was mainly glucose molecules connected with 1,4-glycosidic linkages which contained consid- erable amounts of galactose connected through 1,6-glycosidic linkages. Little cytotoxicity was apparent in cancer cell lines （AGS, DLD-1, HeLa） from the addition of these polysaccharides. However, they could enhance the prolifera- tion of murine macrophage Raw264.7 cells and significantly stimulated Raw264.7 to release nitric oxide （NO） , sug- gesting their potential immunomodulating activities.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2013年第3期38-43,共6页
Food and Fermentation Industries
基金
国家自然科学基金(31201333)
黑龙江省科技厅重点攻关项目(GB07B408)
黑龙江八一农垦大学启动基金(2012YB-11)
黑龙江八一农垦大学大学生创新创业训练计划项目(200266-4022)
关键词
真姬菇
多糖
分子结构
抗癌活性
免疫活性
Hypsizigus marmoreus, polysaccharides, molecular characteristics, anticancer activity, immunomodula-tory activity
