摘要
以莱克多巴胺为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,乙醇为致孔剂采用沉淀聚合法合成了对莱克多巴胺具有高特异识别性的分子印迹聚合物,平衡吸附量达145.6μg/g微球。将制备的印迹聚合物微球装填高效液相色谱柱(50 mm×4.6 mm i.d.)。印迹柱的印迹因子(IF)为1.2,印迹柱和非印迹柱的保留因子(k)分别为33.3和15.1,表明合成聚合物印迹效果明显。分别用10μg/mL克伦特罗、沙丁胺醇和莱克多巴胺3种β-兴奋剂溶液考察自制高效液相色谱柱的分离效果,克伦特罗和沙丁胺醇的选择性系数(α)分别为2.3和1.2,表明所制备的分子印迹柱对以上3种β-兴奋剂显示了良好的选择性。
Molecularly imprinted polymers （MIP） with high selectivity to ractopamine （RAC） were prepared in ethanol using ractopamine as a template, methacrylic acid as a monomer, and ethylene glycol dimethacrylate as a cross-linker by precipitation polymerization, The binding capacity of the polymer toward RAC was found to be about 145.6 μg of ractopamine / g of polymer. The MIP microspheres were packed into high performance liquid chromatographic （HPLC） column （50 mm × 4.6 mmi. d. ）. The imprinted factor （Ir ） of the imprinted column reached 1. 2. The capacity factors （k） of the imprinted column and the non-imprinted column towards ractopamine were calculated as 33.3 and 15.1, respectively. The results showed that there is a good imprinting effect of the imprinted column to ractopamine. Additionally, the selectivity factors （α） of the imprinted column for clenbuterol, salbutamol and ractopamine were evaluated using HPLC. The results showed that the selectivity factors （α） of the column for clenbuterol and salbutamol were 2. 3 and 1.2, respectively. The imprinted column possessed high selectivity to these three β-agonists.
出处
《分析试验室》
CAS
CSCD
北大核心
2013年第5期15-19,共5页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金项目(31072166)
东莞市高等院校科研机构科技项目(201110810200)资助
关键词
分子印迹聚合物
莱克多巴胺
沉淀聚合
色谱表征
Molecularly imprinted polymers
Ractopamine
Precipitation polymerization
Chromatographic characterization
