摘要
目的:观察不同pH值L-精氨酸腹腔注射诱导急性胰腺炎模型的效果。方法:70只BABL/C小鼠,随机分为pH11组、pH8组和对照组,分别采用pH值为11的20%L-精氨酸溶液、pH值为8的20%L-精氨酸溶液、等体积生理盐水,间隔1h 2次小鼠腹腔内注射(250mg/100g体质量)的方法。在24h和48h分别观测三组小鼠的血清淀粉酶、胰腺病理学评分,并统计死亡率。结果:pH11组、pH8组小鼠在24h和48h的血清淀粉酶水平均较对照组显著升高(P<0.05,P<0.01);PH11组较pH8组24h和48h血清淀粉酶水平均升高更明显(P<0.01);pH8组24h和48h胰腺病理评分均较pH11组显著降低(P<0.05),同时该组相应时间点的小鼠死亡率也较后者显著下降(P<0.05)。结论:L-精氨酸溶液的pH值对胰腺损伤程度和动物的死亡率产生很大影响;精氨酸的强碱性对腹腔脏器的损伤是导致模型病变不稳定性的重要原因。
Objective： To investigate the effect of acute pancreatitis model induced by different pH values of L-arginine. Methods： Seventy BABL/C mice were randomly divided into 3 groups. Two groups were treated with L-arginine solution of pH 11 and pH 8,respectively. L-arginine solutions were intraperitoneally injected 250mg/ 100g body weight twice at an interval of 1 hour. Another group served as control and was treated with same vol- ume of saline. Serum amylase was determined and pancreas pathological score and mortality rate were calculated in all groups at the time of 24 and 48 hours after injection. Results： Compared with the control group, the se- rum amylase levels were significantly increased in the pHll group and pH8 group at the time of 24 and 48 hours（P〈0.01）. Compared with the pHll group, the serum amylase levels in the pH8 group were significantly de- creased at the time of 24h and 48h（P〈0.01）, meanwhile, the pancreas pathological score and mortality rate in the pH8 group were also decreased significantly at both time points（P〈0.05）. Conclusion： The pH value of L-ar- ginine solution has a significant impact on the degree of pancreatic injury and animal mortality. The strong alka- line of L-arginine which does damage to abdominal organs is an important reason for instability of acute pancre- as model.
出处
《浙江中西医结合杂志》
2013年第4期265-268,共4页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
浙江省医药卫生科技计划项目(No.2011KYB075)
