摘要
目的研究临床分离嗜麦芽窄食单胞菌的耐药基因分布及分子分型,为分子流行病学研究以及抗感染治疗提供依据。方法采用PCR法对18株嗜麦芽窄食单胞菌进行L1、L2的基因筛查,利用多位点序列分析(MLST)技术进行分子分型研究。结果 18株嗜麦芽窄食单胞菌中L1基因13株,全部具有L2基因。MLST结果显示,18株临床菌株中ST31型6株;ST4型4株;ST25、ST29型各3株;ST8、ST28型各2株;发现两株新型。结论耐药基因L1、L2存在于多数嗜麦芽窄食单胞菌中,可能是导致该菌对β内酰胺类耐药的主要原因。MLST提供了嗜麦芽窄食单胞菌分子流行病学研究的新方法。
Objective Analysing the drug-resisitant gene distribution and molecular typing of Stenotrophomonas ma- ltophilia which isolated from clinical in order to provide the evidence of molecular epidemiological study and anti-infec- tive therapy. Methods Using PCR for the 18 Stenotrophomonas maltophilia strainsgene screening of L1 and L2 ;making the molecular typing analysis by MLST. Results There are 13 strains of Stenotrophomonas maltophilia have L1 gene,all of them have L2 gene.MLST shows that 6 strains have the same ST number 31,4 strains are ST4,3 strains are ST25 and ST29.2 strains are ST number 8 and ST28. Conclusion The drug-resistance gene L1 and L2 consist in vast majority Stenotrophomonas maltophilia,it maybe the main reason of resistant to β-lactam; MLST provide the new method for researching the Molecular epidemiological study of Stenotrophomonas maltophilia.
出处
《中国实验诊断学》
2013年第4期607-610,共4页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金(81071399)
首都医学发展研究基金(2009-3071)
