摘要
目的了解桂西壮族人群蛋氨酸合成酶及其还原酶基因的分布特点。方法应用聚合酶链反应技术对220例桂西壮族健康成年人蛋氨酸合成酶(MS)及蛋氨酸合成酶还原酶(MTRR)基因进行扩增,进行图谱分析。并结合文献进行不同种族间的分析比较。结果桂西壮族人群中MS基因型以AA最多见,AG次之。AA基因型频率为79.09%、AG为19.55%、GG为1.36%。A基因频率为88.86%、G为11.14%。MTRR基因第66位点AA、AG和GG基因型分别为37.73%、53.18%、9.09%,与其他种族相比较,MS基因型在中国正常人群中的分布与白种人群中的分布差异有统计学意义。结论蛋氨酸合成酶及其还原酶基因多态性在不同地域不同种族间分布存在着明显的差异。
Objective To investigate the population distribution feature of methionine synthase and its reductase gene in the west of Guangxi Zhuang Autonomous Region. Methods Application of polymerase chain reaction in 220 cases of healthy adult Zhuang nationality of methionine synthase（MS） and methionine synthase reduetase（MTRR） gene was amplified,for mapping analy- sis. Combined with literature analysis and comparison between different ethnic groups. Results MS genotype in Western Guangxi Zhuang population are most commonly seen in AA, followed by AG. The AA genotype frequency was 79.09%, AG 19.55%, GG 2.36%. The A genotype frequency was 88.86% ,G was 11.14%. MTRR gene sixty-sixth＇loci AA,AG and GG genotypes in the control group were 37.73%,53.18%,9.09%,compared with other races,there are significant differences between the distribution of MS genotypes in Chinese in normal population and Caucasian population. Conclusion Methionine synthase and its reductase gene polymorphism exist obvious differences which in different regions and different ethnic distribution.
出处
《国际检验医学杂志》
CAS
2013年第8期976-976,978,共2页
International Journal of Laboratory Medicine
基金
广西教育厅课题(200810LX087)
关键词
甲硫氨酸
基因型
聚合酶链反应
methionine
genotype
polymerase chain reaction
