摘要
以40%棉粕为主的混合蛋白源制成基础饲料,分别在其中添加0.00%、0.14%、0.28%、0.42%、0.56%蛋氨酸(分别记为0.00%Met、0.14%Met、0.28%Met、0.42%Met和0.56%Met),配制了5种等氮等能的试验饲料,以全鱼粉组(64.4%鱼粉)作为对照,探讨了在高比例棉粕饲料中补充蛋氨酸对中华绒螯蟹幼蟹(0.39±0.02)g的摄食率、生长率和机体抗氧化酶活性的影响。结果表明:与全鱼粉对照组相比,0.42%Met组的增重率、特定生长率和饲料系数均无显著差异(P>0.05),但其显著高于0.00%Met、0.14%Met和0.28%Met组(P<0.05);增重率和特定生长率则显著高于0.56%Met组(P<0.05);从幼蟹的摄食量和蛋白质沉积率来看,0.42%Met和0.28%Met组的摄食量与对照组相似,当蛋氨酸的补充量低于0.28%或高于0.42%时,幼蟹的摄食量均有所下降;统计表明,对照组的蛋白质沉积率最高(23.20%),在各试验组中,0.28%Met和0.42%Met组之间的蛋白质沉积率无显著差异(P>0.05),但显著高于0.00%Met、0.14%Met和0.56%Met组(P<0.05);分析幼蟹的肠道胰蛋白酶活性,发现0.42%Met组与对照组之间无显著性差异(P>0.05),但均显著高于其他各试验组(P<0.05);0.28%Met、0.42%Met和0.56%Met处理组的血清丙二醛含量、超氧化物歧化酶活性和谷胱甘肽过氧化物酶活性与对照组无显著差异(P>0.05)。结果提示,在高比例植物蛋白(40%棉粕)的基础饲料中,补充0.42%Met能够显著提高中华绒螯蟹幼蟹的生长率、消化率和抗氧化酶的活性。
This study was conducted to evaluate the effects of dietary methionine supplementation in cottonseed mealbased diets on the growth performance,digestibility and antioxidant ability of juvenile Chinese mitten crab,Eriocheir sinensis.Five diets were formulated using a basic diet,in which cottonseed meal accounted for 40%,supplemented with 0.00%,0.14%,0.28%,0.42%,and 0.56% methionine,respectively.Another diet was full fish meal-based（fish meal accounted for 64.4%） and served as the control.Each diet was fed to juvenile E.sinensis [（0.39±0.02） g] of five replicates for 6 weeks.The results showed that weight gain,specific growth rate and feed conversion ratio in crabs fed 0.42%Met diet did not differ from those of the control group（P0.05）.Crabs fed with 0.28%Met and 0.42% Metdiets exhibited higher food intake and protein deposition rate than those fed diets with 0.00%Met,0.14%Met,0.56%Met（P0.05）.There were no significant differences in whole body moisture,lipid,and ash（P0.05） among all treatments.However,compared with the 0.00% Met diets group,crude protein contents in crab fed with methionine supplemented diets were significantly higher（P0.05）.Dry matter,protein,lipid digestibility,and trypsinase activity of crabs fed with 0.42% Metdiets did not differ from those of the control（P0.05）.No differences were found in malondiaedehyde content,serum superoxide dismutase and glutathione peroxidase activity in crabs fed with the control diet and those fed with 0.28% Met,0.42%Met and 0.56% Metdiets（P0.05）.The results suggest that 0.42%Met added to the diet（cottonseed meal accounted for 40%） could significantly accelerate the growth performance,digestibility and antioxidant ability of juvenile E.sinensis.
出处
《水生生物学报》
CAS
CSCD
北大核心
2013年第2期336-343,共8页
Acta Hydrobiologica Sinica
基金
国家自然科学基金项目(No.31172422)
公益性行业(农业)科研专项(No.201003020
201203065)
国家基础研究973计划(2009CB118702)
"十二五"国家科技支撑计划课题(2012BAD25B00)
上海市中华绒螯蟹现代农业产业技术体系建设和上海市科委重点项目(No.10JC1404100)部分资助
关键词
中华绒螯蟹
蛋氨酸
棉粕替代
摄食
生长
抗氧化酶
Eriocheir sinensis
Methionine
Cottonseed meal
Feeding
Growth
Antioxidant
