摘要
目的观察常频机械通气（CMV）、高频振荡通气（HFOV）、部分液体通气（PLV）3种机械通气方式对急性肺损伤（ALI）新生猪BALF炎性因子及肺表面活性物质相关蛋白A（SP—A）水平的影响。方法出生1～3d健康新生猪24只，用9g／L盐水（38℃，35mL／kg）灌洗制备ALI模型。随机分为4组：对照组（6只，模型制备成功后不予通气，直接处死）、CMV组（6只）、HFOV组（6只）、PLV组（6只），行机械通气24h后处死动物，用ELISA法检测BALF中TNF-α、IL-8、IL-1及sP—A水平。结果3种方式机械通气24h后，BALF中3种炎性因子及SP—A总体均数比较差异均有统计学意义（P均=0．000），PLV组、HFOV组sP-A水平较CMV组高（P均〈0．05），PLV组IL培、IL-1、TNF-α水平较CMV组低（P均〈0．05），PLV组IL-8、TNF-α水平较HFOV组低（P均〈0．05），HFOV组IL-8、TNF-α水平较CMV组低（P均〈0．05）。结论不同机械通气方式致肺部炎性反应不同，PLV致肺部炎性反应最轻。PLV较CMV、HFOV更能增加SP—A表达，降低SP—A的降解。
Objective To investigate the effects of 3 different ventilation methods,including conventional me- chanical ventilation （CMV）, high frequency oscillatory ventilation（ HFOV ）and partial liquid ventilation （PLV）, on the changes of inflammatory factors and pulmonary surfactant associated protein A （ SP-A ） in bronchoalveolar lavage fluid （BALF） in newborn piglets with acute lung injury （ALI）. Methods Twenty-four newborn piglets, no more than 3 days old,were enrolled. After ALI made with saline lavage （38 ℃ ,35 mL/kg） , newborn piglets were randomly assigned to 4 groups ： control group （ n = 6, no ventilation）, CMV group （ n = 6 ）, HFOV group （ n = 6 ）, and PLV group （ n = 6 ）. Piglets were sacrificed after being ventilated for 24 h. Tumor necrosis factor-α （ TNF-α）, interleukin-8 （ IL-8 ） , interleukin-1 （ IL- l ） and SP-A in BALF were measured quantitatively by using enzyme-linked immunosorbent assay. Results In 3 groups using different ventilation methods, the population mean of TNF-α, IL-8, IL-1 and SP-A were statistically diffe- rent（ all P = 0. 000 ）. SP-A in PLV group and HFOV group were higher than that in CMV group （all P 〈 0.05 ）, while IL-8, IL-1 and TNF-α in PLV group were lower than those in CMV group （ all P 〈 0.05 ）, IL-8 and TNF-α in PLV group were lower than those in HFOV group （ all P 〈 0.05 ）, IL-8 and TNF-α in HFOV group were lower than those in CMV group （ all P 〈 0.05 ）. Conclusions Pulmonary inflammatory reaction was different in 3 ventilation groups. Com- pared with CMV and HFOV, PLV attenuated inflammatory reaction, so it could increase the expression of SP-A and de- crease the degradation of SP-A.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2013年第6期411-413,共3页
Chinese Journal of Applied Clinical Pediatrics
基金
广东省科技计划项目（20088030301164）
关键词
机械通气方式
支气管肺泡灌洗液
炎性因子
肺表面活性物质相关蛋白A
Mechanical ventilation method
Bronchoalveolar lavage fluid
Inflammatory factor
Pulmonary sur- factant associated protein A
