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辐照灭菌用于纳豆激酶制剂的实验研究

The Experiment Study of the γ Radiation on the Nattokinase Crudes
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摘要 对60钴γ-射线辐照前后的纳豆激酶(NK)制剂有效成分、酶活及活菌数等技术指标进行了实验研究,结果表明,不同剂量的60钴γ-射线辐照对纳豆激酶制剂中的总皂甙含量和纳豆激酶酶活影响不大,30 kGy时总皂甙含量仍可达到原始含量的105%~125%,NK酶活达到原始酶活的90%以上;纳豆芽孢杆菌、大肠菌群和金黄色葡萄球菌等活菌数对60钴γ-射线辐照十分敏感,5 kGy的60钴γ-射线辐照处理后纳豆芽孢杆菌、大肠菌群和金黄色葡萄球菌数量下降99%以上. The nattokinase etudes were treated with Y-ray irradiation at different absorbed doses, and the total ginsenosides, nattokinase activity and the vital microbes were tested. It was found that the influences of the Y-ray irradiation on the total ginsenosides and nattokinase activity were slightly,while the lethal effect of Y-ray irradiation on the vital microbes such as Bacillus subtilus Natto,Staphylococcus aureus and coliform bacteria were seriously (decreased 99%). This studies provided some experimental data for irradiation methods applicated on the nattokinase crudes disinfection and preservation.
出处 《河南科学》 2013年第4期441-444,共4页 Henan Science
基金 河南省高新技术产业化项目(112105000030)
关键词 γ-射线辐照 纳豆激酶酶活 活菌数 灭菌 Y-ray irradiation nattokinase fibrinolytic activity vital microbes disinfection
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