摘要
目的探讨多发性骨髓瘤(MM)患者外周血T细胞亚群和CD4+CD25+调节性T(Treg)细胞的表达特点,分析MM与健康者之间的细胞免疫功能差异。方法检测28例初诊的MM患者及22例健康志愿者外周血淋巴细胞中总T细胞(CD3+)、辅助/诱导T细胞(CD3+CD4+)、抑制/细胞毒T细胞(CD3+CD8+)的比例、(CD3+CD4+)/(CD3+CD8+)、CD4+CD25+/CD4+、CD4+CD25+FoxP3+/CD4+CD25+比值,并比较MM患者与健康者之间细胞免疫指标(Th1/Th2比值、AgNOR比值、TGF-β)的差异。结果初诊MM患者与对照组相比,外周血淋巴细胞中总T细胞(CD3+)比例、辅助/诱导T细胞(CD3+CD4+)比例差异无统计学意义(P>0.05);抑制/细胞毒T细胞(CD3+CD8+)比例增加(P<0.05);CD4+CD25+/CD4+、CD4+CD25+FoxP3+/CD4+CD25+比值明显高于健康组(P<0.05);血清中Th1/Th2比值、AgNOR比值均明显低于健康组(P<0.05);血清TGF-β含量较健康组上升(P<0.01)。结论 MM患者外周血T细胞亚群的异常、Treg表达增加及细胞免疫功能低下可能与疾病的发生、发展相关,对判断MM疗效及预后有一定的指导价值。
Objective To study the characteristics of T lymphocyte subsets,CD4+CD25+Treg cells in peripheral blood from the patients with multiple myeloma(MM) and analyse the differences of cellular immune functions between the MM and the healthy persons.Methods The proportions of CD3+,CD3+CD4+,CD3+CD8+cells in peripheral blood and the ratios of CD3+CD4+/CD3+CD8+,CD4+CD25+/CD4+,CD4+CD25+FoxP3+/CD4+CD25+ were analyzed between the 28 patients with MM in newly diagnosed and 22 healthy volunteers(control group) and the differences of cellular immune indicators(Th1/Th2,the ratio of AgNOR,and TGF-β) between the two groups were compared.Results Compared with the healthy people,the proportions of CD3+、CD3+CD4+cells in lymphocyte from the patients with multiple myeloma in newly diagnosed were no significance respectively(P>0.05),and the proportion of CD3+CD8+cells in lymphocyte from the patients with multiple myeloma in newly diagnosed was higher than those of the control group(P<0.05).The ratios of CD4+CD25+/CD4+ and CD4+CD25+FoxP3+/CD4+CD25+ from the cases in newly diagnosed were clearly higher than those of control group(P<0.05).The Th1/Th2 and the ratio of AgNOR in serum from the patients were significantly lower than the healthy group(P<0.05).The level of TGF-β in serum from the patients was higher than the healthy group(P<0.01).Conclusion The abnormality of T cell subsets in peripheral blood and the excessive expression of Tregs and the cellular immune function were related with the occurrence and development of disease.So it had certain clinical instructional value in judging the therapy and prognosis of the patients with multiple myeloma.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第11期1210-1212,共3页
Chongqing medicine
基金
四川省卫生厅项目(090173)
