摘要
目的:优选复方止颤汤的提取工艺。方法:采用HPLC测定钩藤碱、异钩藤碱、芍药苷含量;以钩藤碱、异钩藤碱、芍药苷提取率为指标,通过L9(34)正交试验考察加水量、提取时间、提取次数对提取工艺的影响。结果:最佳提取工艺条件为加10倍量水提取2次,每次1 h。结论:优选的止颤汤提取工艺稳定可行,提取率高。
Objective：To optimize extraction technology of compound Zhichan decoction.Method： With the content of rhynchophylline,isorhynchophylline,paeoniflorin as index,which were determined by HPLC,effects of the amount of water,extraction time,extraction times on extraction technology was investigated by L9（34） orthogonal test.Result： Optimal extraction technology conditions were as followings：extracted 2 times with 10 folds the amount of water,1 h per time.Conclusion： This optimized extraction technology was stable and feasible with high extraction rate.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第9期21-24,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
上海市科委中药现代化专项(10DZ1973000)
上海高校选拔培养优秀青年教师科研专项基金(szy09006)
上海市自然科学基金项目(09ZR1429600)
上海市教委创新项目(10YZ57)
上海市教委重点学科项目(J50302)
关键词
正交试验
提取工艺
止颤汤
钩藤碱
异钩藤碱
芍药苷
orthogonal test
extraction process
Zhichan decoction
rhynchophylline
isorhynchophylline
paeoniflorin
